Yoon Y S, Noma T, Yamashiro Y, Ito H, Nakazawa A
Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Neurosci Lett. 1998 Jul 17;251(1):17-20. doi: 10.1016/s0304-3940(98)00490-x.
NeuroD is a basic helix-loop-helix transcription factor that binds to an E-box sequence, CANNTG, in the target gene promoter region. The gene encoding NeuroD is expressed specifically in brain, intestine, and pancreatic cells and plays a pivotal role in tissue-specific differentiation. To investigate the regulation of human NeuroD gene expression, we isolated and sequenced a genomic DNA containing two exons and flanking regions of the NeuroD gene. The sizes of exons 1 and 2 were 168 and 2404 bp, respectively. CAT reporter analysis showed that the elements responsible for basal promoter activity lay in the proximal 408-bp region. Cotransfection of the CAT reporter plasmids with a NeuroD expression plasmid resulted in 5-fold enhancement of the CAT activity, indicating an autoregulatory mechanism is involved in human NeuroD gene expression.
NeuroD是一种碱性螺旋-环-螺旋转录因子，它能与靶基因启动子区域的E-box序列CANNTG结合。编码NeuroD的基因在脑、肠和胰腺细胞中特异性表达，并在组织特异性分化中起关键作用。为了研究人类NeuroD基因表达的调控机制，我们分离并测序了一段包含NeuroD基因两个外显子及其侧翼区域的基因组DNA。外显子1和2的大小分别为168 bp和2404 bp。CAT报告基因分析表明，负责基础启动子活性的元件位于近端408 bp区域。将CAT报告质粒与NeuroD表达质粒共转染导致CAT活性增强5倍，表明人类NeuroD基因表达涉及一种自调控机制。
