Monji A, Tashiro K, Hayashi Y, Yoshida I, Tashiro N
Department of Neuropsychiatry, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Neurosci Lett. 1998 Jul 17;251(1):65-8. doi: 10.1016/s0304-3940(98)00499-6.
We investigated whether or not laminin 1 and the two different synthetic peptides deduced from the sequence in the laminin alphal chain, both of which mediate cell attachment and neurite outgrowth in PC12 cells, have an effect on Abeta40 fibril formation in vitro. A thioflavine-T fluorometric assay showed a synthetic peptide containing the YFQRYLI sequence from the laminin alpha1 chain to inhibit Abeta40 fibril formation while the inhibitory effect of this peptide was found to be somewhat less than that of intact laminin 1. These results were confirmed by electron microscopic observations using negative staining. The findings of the present study suggested that the synthetic peptide derived from the laminin alpha1 chain may thus be an effective therapeutic agent for either preventing or slowing down the progression of amyloidogenesis in Alzheimer's disease.
我们研究了层粘连蛋白1以及从层粘连蛋白α1链序列推导出来的两种不同合成肽（二者均可介导PC12细胞的细胞黏附和神经突生长）是否对体外β-淀粉样蛋白40（Aβ40）纤维形成有影响。硫黄素-T荧光测定显示，一种含有来自层粘连蛋白α1链YFQRYLI序列的合成肽可抑制Aβ40纤维形成，不过该肽的抑制作用略小于完整的层粘连蛋白1。这些结果通过负染色电子显微镜观察得到证实。本研究结果表明，源自层粘连蛋白α1链的合成肽可能是预防或减缓阿尔茨海默病淀粉样蛋白生成进程的有效治疗剂。
