Bresciani A, Pirozzi G, Spera M, Lombardi M L, Ambrosone L, Migliaresi S, Ferrone S, Manzo C
Servizio di Oncologia Sperimentale C-Immunologia, Istituto Tumori Fondazione Pascale, Napoli, Italy.
Tissue Antigens. 1998 Jul;52(1):44-50. doi: 10.1111/j.1399-0039.1998.tb03022.x.
The level of soluble beta2-mu-associated HLA Class I heavy chains (sHLA-I) and of soluble beta2-mu-free HLA Class I heavy chains (sHLA-FHC) was found to be significantly higher in sera from 58 patients with systemic lupus erythematosus (SLE) than in those from 82 age and sex-matched controls. The level of serum sHLA-I in patients with SLE was significantly correlated to disease activity. Western blotting analysis showed that the 44-kDa isoform represents the major component in the antigens immunoprecipitated by anti-beta2-mu mAb NAMB-1 and by anti-beta2-mu-free HLA Class I heavy chain mAb HC-10 from sera of patients with SLE. These results suggest that the increased serum levels of sHLA-I and of sHLA-FHC in patients with SLE reflect their increased shedding from cell membrane. In view of the ability of sHLA-I and of sHLA-FHC to induce apoptosis of activated T cells, it is suggested that their increased serum levels in patients with SLE is triggered by dysregulation of the immune system leading to T-cell activation. The increased serum levels of sHLA-I and of sHLA-FHC may be used by the immune system to control the pool of activated T cells by inducing apoptosis. If this possibility is proven to be correct, modulation of the serum level of sHLA-I and of sHLA-FHC may be utilized to develop strategies to treat SLE.
研究发现，58例系统性红斑狼疮（SLE）患者血清中可溶性β2-微球蛋白相关的HLA I类重链（sHLA-I）和可溶性无β2-微球蛋白的HLA I类重链（sHLA-FHC）水平显著高于82例年龄和性别匹配的对照组。SLE患者血清sHLA-I水平与疾病活动度显著相关。蛋白质印迹分析表明，44 kDa异构体是抗β2-微球蛋白单克隆抗体NAMB-1和抗无β2-微球蛋白的HLA I类重链单克隆抗体HC-10从SLE患者血清中免疫沉淀的抗原中的主要成分。这些结果表明，SLE患者血清中sHLA-I和sHLA-FHC水平升高反映了它们从细胞膜上脱落增加。鉴于sHLA-I和sHLA-FHC能够诱导活化T细胞凋亡，提示SLE患者血清中它们水平升高是由免疫系统失调导致T细胞活化引起的。血清中sHLA-I和sHLA-FHC水平升高可能被免疫系统用于通过诱导凋亡来控制活化T细胞库。如果这种可能性被证明是正确的，调节血清sHLA-I和sHLA-FHC水平可用于开发治疗SLE的策略。
