一种粟酒裂殖酵母基因，编码一种具有预测的α-螺旋杆状结构的新型多肽，该结构存在于肌球蛋白和中间丝蛋白家族中。

A Schizosaccharomyces pombe gene encoding a novel polypeptide with a predicted alpha-helical rod structure found in the myosin and intermediate-filament families of proteins.

作者信息

Jannatipour M, Rokeach L A

机构信息

Département de Biochimie, Université de Montréal, Quebec, Canada.

出版信息

Biochim Biophys Acta. 1998 Jul 30;1399(1):67-72. doi: 10.1016/s0167-4781(98)00097-9.

DOI:10.1016/s0167-4781(98)00097-9

PMID:9714741

Abstract

We have identified a Schizosaccharomyces pombe gene encoding a 461 amino acid polypeptide containing a predicted alpha-helical rod domain found in filamentous proteins. This gene, here designated noc1, is located immediately upstream from cnx1, the gene encoding the S. pombe homologue of mammalian calnexin, an endoplasmic reticulum chaperone [M. Jannatipour, L.A. Rokeach, J. Biol. Chem. 270 (1995) 4845-4853.]. Transcription of noc1 is divergent from that of cnx1. Northern blot analysis identified a single mRNA of approx. 2 kb whose expression was increased by heat shock and growth in the presence of beta-mercaptoethanol and 2-deoxyglucose.

摘要

我们已经鉴定出一个粟酒裂殖酵母基因，该基因编码一个含有461个氨基酸的多肽，其中包含在丝状蛋白中发现的预测α-螺旋杆结构域。这个基因，在这里命名为noc1，位于cnx1的紧邻上游，cnx1是编码哺乳动物钙连蛋白（一种内质网伴侣蛋白）的粟酒裂殖酵母同源物的基因[M. Jannatipour, L.A. Rokeach, J. Biol. Chem. 270 (1995) 4845 - 4853]。noc1的转录方向与cnx1相反。Northern印迹分析鉴定出一条约2 kb的单一mRNA，其表达在热休克以及在β-巯基乙醇和2-脱氧葡萄糖存在的情况下生长时会增加。