Xp21.3-p11.3区域15兆碱基的酵母人工染色体/序列标签位点图谱，分辨率为100千碱基，对遗传距离和杜氏肌营养不良症结构进行了精细比较。

YAC/STS map of 15Mb of Xp21.3-p11.3, at 100kb resolution, with refined comparisons of genetic distances and DMD structure.

作者信息

Nagaraja R, Jermak C, Trusgnich M, Yoon J, MacMillan S, McCauley M B, Brownstein B, Schlessinger D

机构信息

Washington University School of Medicine, Center for Genetics in Medicine, 660 South Euclid Avenue, Box 8232, St. Louis, MO 63110, USA.

出版信息

Gene. 1998 Jul 30;215(2):259-67. doi: 10.1016/s0378-1119(98)00293-5.

DOI:10.1016/s0378-1119(98)00293-5

PMID:9714824

Abstract

The 15Mb region between DXS997 and DXS8054 in Xp21.3-p11.3 has been mapped at seven-fold average coverage in yeast artificial chromosomes (YACs) and 100 kb inter-sequence tagged site (STS) distance. YACs from six different collections show self-consistent maps. The STSs include 18 (CA) repeat and one tetranucleotide repeat marker that detect polymorphism, as well as eight well-studied genes, a second site for MXS1 sequences, and three expressed sequence tags (ESTs). One of the ESTs maps to intron 7 of Duchenne muscular dystrophy, and seems to be a processed intronic sequence with a poly(A) tail.

摘要

位于Xp21.3 - p11.3区域中DXS997和DXS8054之间的15Mb区域已在酵母人工染色体（YAC）中以平均7倍的覆盖率以及100kb的序列标签位点（STS）间隔进行了图谱绘制。来自六个不同文库的YAC显示出相互一致的图谱。这些STS包括18个（CA）重复序列和1个可检测多态性的四核苷酸重复标记，以及8个经过充分研究的基因、MXS1序列的第二个位点和3个表达序列标签（EST）。其中一个EST定位于杜兴氏肌营养不良症基因的第7内含子，似乎是一个带有poly(A)尾的加工后的内含子序列。