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选择用于麻风分枝杆菌染色的脱色剂及其浓度。这真的重要吗?

Choosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter?

作者信息

de Soldenhoff R, Hatta M, Siro T W

机构信息

NSL-South Sulawesi Leprosy Control Project, Provincial Health Services, South Sulawesi, Indonesia.

出版信息

Lepr Rev. 1998 Jun;69(2):128-33. doi: 10.5935/0305-7518.19980013.

DOI:10.5935/0305-7518.19980013
PMID:9715597
Abstract

Leprosy bacilli are more easily decolourized during staining than tuberculosis bacilli, so a weaker concentration of decolourizer is usually recommended. In Indonesia, the same 'strong' decolourizer is used for identifying both organisms. In a study to compare the results using different concentrations of different decolourizers, no difference could be found in the bacterial index (BI). It is suggested that the same staining technique can be used for tuberculosis and leprosy.

摘要

麻风杆菌在染色过程中比结核杆菌更容易脱色,因此通常建议使用浓度较低的脱色剂。在印度尼西亚,使用相同的“强效”脱色剂来鉴定这两种细菌。在一项比较使用不同浓度的不同脱色剂的结果的研究中,细菌指数(BI)没有差异。有人认为,相同的染色技术可用于结核病和麻风病。

相似文献

1
Choosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter?选择用于麻风分枝杆菌染色的脱色剂及其浓度。这真的重要吗?
Lepr Rev. 1998 Jun;69(2):128-33. doi: 10.5935/0305-7518.19980013.
2
Two methods of demonstrating leprosy bacilli in smears.在涂片上显示麻风杆菌的两种方法。
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Elimination of the acid fastness but not the gram positivity of leprosy bacilli after extraction with pyridine.用吡啶提取后,麻风杆菌的抗酸性消失,但革兰氏阳性特性未消失。
J Bacteriol. 1971 May;106(2):707-8. doi: 10.1128/jb.106.2.707-708.1971.
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The technique of staining leprosy bacilli in smears.涂片上麻风杆菌的染色技术。
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[Morphologic structure of Mycobacterium leprae].[麻风分枝杆菌的形态结构]
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Morphology of Mycobacterium leprae in tissue sections. Correlation with results of mouse foot pad inoculation.组织切片中麻风分枝杆菌的形态学。与小鼠足垫接种结果的相关性。
Arch Dermatol. 1969 Nov;100(5):618-20.
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The use of non-deparaffinized tissue sections for staining leprosy bacilli.使用非脱蜡组织切片对麻风杆菌进行染色。
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