人脐带血中树突状细胞的功能活性

Functional competence of dendritic cells in human umbilical cord blood.

作者信息

Sorg R V, Kögler G, Wernet P

机构信息

Bone Marrow Donor Center with Transplantation Immunology and EuroCord Bank Germany, Heinrich Heine University Medical Center, Düsseldorf.

出版信息

Bone Marrow Transplant. 1998 Jul;22 Suppl 1:S52-4.

PMID:9715887

Abstract

Dendritic cells (DC) are the main stimulators of primary T cell responses. Very little is known about DC in cord blood (CB), and whether they are involved in the low incidence and severity of GVHD following CB transplantation. Here, CBDC were identified as a HLA-DR+/lineage marker (lin; CD3, CD11b, CD14, CD16, CD19, CD34, CD56 and glycophorin A antigens) negative population, representing 0.3 +/- 0.1% (mean +/- s.d.; n = 15) of CB mononuclear cells. CBDC expressed the CD4, CD11a, CD18, CD45RA, CD50 and CD54 antigens but revealed no expression of the CD1a, CD11c, CD40, CD45R0, CD58, CD83, CD86 and CD102 antigens. Immunomagnetically enriched CBDC showed potent allostimulatory activity for CB T cells. Thus, CBDC are functionally competent and resemble in their immature/resting state CD11c- DC in peripheral blood.

摘要

树突状细胞（DC）是初始T细胞反应的主要刺激物。关于脐血（CB）中的DC以及它们是否参与CB移植后移植物抗宿主病（GVHD）的低发病率和低严重程度，人们了解甚少。在这里，脐血树突状细胞（CBDC）被鉴定为HLA - DR + /谱系标志物（lin；CD3、CD11b、CD14、CD16、CD19、CD34、CD56和血型糖蛋白A抗原）阴性群体，占CB单个核细胞的0.3 +/- 0.1%（平均值 +/- 标准差；n = 15）。CBDC表达CD4、CD11a、CD18、CD45RA、CD50和CD54抗原，但未检测到CD1a、CD11c、CD40、CD45R0、CD58、CD83、CD86和CD102抗原的表达。免疫磁珠富集的CBDC对CB T细胞显示出强大的同种异体刺激活性。因此，CBDC功能正常，在未成熟/静止状态下类似于外周血中的CD11c - DC。