Denning-Kendall P A, Horsley H, Nicol A, Nieda M, Bradley B, Hows J M
University of Bristol, Division of Transplantation Sciences, UK.
Bone Marrow Transplant. 1998 Jul;22 Suppl 1:S63-5.
Expansion of cord blood (CB) haemopoietic cells has been investigated with the aim of reducing cytopenia following transplantation. We investigated the increase in total nucleated cells, colony-forming cells (CFC), CD34+ cells and long-term culture-initiating cells (LTC-IC) by limiting dilution after a 14-day culture of CB CD34+ cells (5 x 10(3)/ml) with SCF, IL-3, IL-6, GM-CSF and G-CSF all at 10 ng/ml. On average nucleated cells increased 2500-fold, CD34+ cells 39-fold and CFU-GM 49-fold with maintenance of BFU-E. The more primitive LTC-IC expanded on average 2.5-fold. Expansion of a 20% aliquot of a CB donation could provide a 5-7-fold increase in progenitor cells, and a 1570-fold increase in post-progenitor cells compared to an untreated donation.
为了减少移植后的血细胞减少症，人们对脐血（CB）造血细胞的扩增进行了研究。我们研究了在含有10 ng/ml的干细胞因子（SCF）、白细胞介素-3（IL-3）、白细胞介素-6（IL-6）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）的条件下，对CB CD34+细胞（5×10³/ml）进行14天培养后，通过有限稀释法检测总核细胞、集落形成细胞（CFC）、CD34+细胞和长期培养起始细胞（LTC-IC）的增加情况。平均而言，核细胞增加了2500倍，CD34+细胞增加了39倍，粒-巨噬细胞集落形成单位（CFU-GM）增加了49倍，同时保持了爆式红系集落形成单位（BFU-E）。更原始的LTC-IC平均扩增了2.5倍。与未处理的脐血捐献相比，脐血捐献20%的等分样本扩增后可使祖细胞增加5至7倍，祖细胞后细胞增加1570倍。
