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CD45部分介导小鼠白血病/淋巴瘤细胞系L5178Y与骨髓基质细胞之间的异型黏附。

CD45 partially mediates heterotypic adhesion between murine leukemia/lymphoma cell line L5178Y and marrow stromal cells.

作者信息

Juneja H S, Schmalstieg F C, Lee S, Chen J

机构信息

Department of Internal Medicine, University of Texas Health Science Center, Houston 77030, USA.

出版信息

Leuk Res. 1998 Sep;22(9):805-15. doi: 10.1016/s0145-2126(98)00070-8.

Abstract

We raised mAbs to whole L5178Y leukemia/lymphoma (LL) cells to identify adhesion proteins involved in adherence between LL cells and marrow stromal cells. One mAb, 4C, and its subclones 4C.1 and 4C.2 inhibited adherence of L5178Y LL cells to MLT. a nontransformed murine marrow stromal cell line. These MoAbs are directed against CD45RA. Control anti-CD45 mAbs and isotype mAbs were non-inhibitory. Other anti-CD45 mAbs, M1/9.3, RA3-3A1/6.1 and RA3-2C2/1 do not compete with mAb 4C.1 for binding to the L5178Y cell surface, but mAb 4C.1 competes for binding of mAb RA3-2C2/1. Effects of mAb 4C on tyrosine-phosphatase activity of CD45 in L5178Y cells are minimal, suggesting direct involvement of CD45 as an adhesion protein.

摘要

我们制备了针对整个L5178Y白血病/淋巴瘤(LL)细胞的单克隆抗体(mAb),以鉴定参与LL细胞与骨髓基质细胞黏附的黏附蛋白。一种单克隆抗体4C及其亚克隆4C.1和4C.2可抑制L5178Y LL细胞与非转化的小鼠骨髓基质细胞系MLT的黏附。这些单克隆抗体针对CD45RA。对照抗CD45单克隆抗体和同型单克隆抗体无抑制作用。其他抗CD45单克隆抗体M1/9.3、RA3-3A1/6.1和RA3-2C2/1不与单克隆抗体4C.1竞争结合L5178Y细胞表面,但单克隆抗体4C.1与单克隆抗体RA3-2C2/1竞争结合。单克隆抗体4C对L5178Y细胞中CD45酪氨酸磷酸酶活性的影响最小,提示CD45作为黏附蛋白直接参与其中。

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