Tillett M L, Lian L Y, Norwood T J
Department of Biochemistry, Leicester University, University Road, Leicester, LE1 7RH, United Kingdom.
J Magn Reson. 1998 Aug;133(2):379-84. doi: 10.1006/jmre.1998.1472.
NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme.
核磁共振扩散测量已被证明是研究蛋白质构象和寡聚状态的有用工具。文中指出了在水溶液中对蛋白质进行扩散测量时存在的四个主要问题，并提出了解决方案。以鸡蛋清溶菌酶水溶液为例展示了由此得出的实验。
