从人血单核细胞体外分化成熟树突状细胞。
In-vitro differentiation of mature dendritic cells from human blood monocytes.
作者信息
Gieseler R, Heise D, Soruri A, Schwartz P, Peters J H
机构信息
Department of Immunology, Georg-August University, Göttingen, Germany.
出版信息
Dev Immunol. 1998;6(1-2):25-39. doi: 10.1155/1998/72054.
Representing the most potent antigen-presenting cells, dendritic cells (DC) can now be generated from human blood monocytes. We recently presented a novel protocol employing GM-CSF, IL-4, and IFN-gamma to differentiate monocyte-derived DC in vitro. Here, such cells are characterized in detail. Cells in culture exhibited both dendritic and veiled morphologies, the former being adherent and the latter suspended. Phenotypically, they were CD1a-/dim, CD11a+, CD11b++, CD11c+, CD14dim/-, CD16a-/dim, CD18+, CD32dim/-, CD33+, CD40+, CD45R0+, CD50+, CD54+, CD64-/dim, CD68+, CD71+, CD80dim, CD86+/++, MHC class I++/ , HLA-DR++/ , HLA-DP+, and HLA-DQ+. The DC stimulated a strong allogeneic T-cell response, and further evidence for their autologous antigen-specific stimulation is discussed. Although resembling a mature CD11c+ CD45R0+ blood DC subset identified earlier, their differentiation in the presence of the Th1 and Th2 cytokines IFN-gamma and IL-4 indicates that these DC may conform to mature mucosal DC.
作为最有效的抗原呈递细胞，树突状细胞（DC）现在可以从人血单核细胞中产生。我们最近提出了一种使用粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-4（IL-4）和γ干扰素（IFN-γ）在体外分化单核细胞来源的DC的新方案。在此，对这类细胞进行了详细表征。培养的细胞呈现出树突状和面纱状形态，前者贴壁，后者悬浮。从表型上看，它们是CD1a- /弱阳性、CD11a+、CD11b++、CD11c+、CD14弱阳性/阴性、CD16a- /弱阳性、CD18+、CD32弱阳性/阴性、CD33+、CD40+、CD45R0+、CD50+、CD54+、CD64- /弱阳性、CD68+、CD71+、CD80弱阳性、CD86+++、MHC I类++ / 、HLA-DR++ / 、HLA-DP+和HLA-DQ+。这些DC刺激了强烈的同种异体T细胞反应，并讨论了它们自体抗原特异性刺激的进一步证据。尽管它们类似于先前鉴定的成熟CD11c+ CD45R0+血液DC亚群，但在Th1和Th2细胞因子IFN-γ和IL-4存在下的分化表明这些DC可能符合成熟的黏膜DC。
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