Varas A, Romo T, Jiménez E, Alonso L, Vicente A, Zapata A G
Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
Dev Immunol. 1998;6(1-2):141-7. doi: 10.1155/1998/95861.
On the basis of both the interleukin-2-receptor (IL-2R) alpha-chain expression on 16-day-old fetal rat thymocytes and the occurrence of interleukin-2 (IL-2) mRNA-containing cells early during rat thymus ontogeny, we have investigated the possible role of IL-2/IL-2R complex in rat T-cell maturation. For this purpose, we analyzed the effects of the addition of either recombinant rat IL-2 or anti-CD25 (OX-39)-blocking monoclonal antibodies to fetal thymus organ cultures (FTOC), established from 16-day-old rat embryos. IL-2 stimulated the growth of thymocytes and, as a result, induced T-cell differentiation, whereas OX-39 mAb blocked the maturation of thymic-cell progenitors. Accordingly, these results support the involvement of IL-2/IL-2R complex in rat T-cell development.
基于16日龄胎鼠胸腺细胞上白细胞介素-2受体（IL-2R）α链的表达以及大鼠胸腺发育早期含白细胞介素-2（IL-2）mRNA细胞的出现，我们研究了IL-2/IL-2R复合物在大鼠T细胞成熟中的可能作用。为此，我们分析了向取自16日龄大鼠胚胎的胎胸腺器官培养物（FTOC）中添加重组大鼠IL-2或抗CD25（OX-39）阻断单克隆抗体的效果。IL-2刺激胸腺细胞生长，从而诱导T细胞分化，而OX-39单克隆抗体则阻断胸腺细胞祖细胞的成熟。因此，这些结果支持IL-2/IL-2R复合物参与大鼠T细胞发育。
