Johnson E H, Schynder-Candrian S, Rajan T V, Nelson F K, Lustigman S, Abraham D
Section of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520-8016, USA.
Parasite Immunol. 1998 Jul;20(7):319-24. doi: 10.1046/j.1365-3024.1998.00148.x.
To shed clarity on the dichotomy of reported results relative to the significance of T helper-1 vs T helper-2 immune responses in onchocerciasis, we compared the survivability of Onchocerca volvulus third-stage larvae (L3) in immunized mice that had either a targeted disruption of the Interleukin-4 or Interferon-gamma gene. Treatment groups consisted of control mice and mice immunized with irradiated O. volvulus L3. All mice were challenged with diffusion chambers containing viable L3. Vaccinated IL-4-/- were unable to kill this larval target. In contrast, vaccinated INF-gamma-/- and C57BL/6 mice, exhibited high levels of killing, had elevated levels of IL-4 and significantly greater numbers of eosinophils in their diffusion chambers than the IL-4-/-. Whereas, levels of IFN-gamma in all three groups of immunized mice were equivalent to those of control mice, levels of IL-5 were elevated, even in the IL-4-/-, indicating that cytokines other than IL-4 were involved in its production. The protective immune response to third-stage larvae of O. volvulus in mice vaccinated with irradiated larvae has an absolute IL-4 requirement.
为了阐明盘尾丝虫病中所报道结果的二分法与辅助性T细胞1型（Th1）和辅助性T细胞2型（Th2）免疫反应的重要性之间的关系，我们比较了盘尾丝虫第三期幼虫（L3）在白细胞介素-4（IL-4）或干扰素-γ基因靶向破坏的免疫小鼠中的生存能力。治疗组包括对照小鼠和用辐照过的盘尾丝虫L3免疫的小鼠。所有小鼠均用含有活L3的扩散室进行攻击。接种疫苗的IL-4基因敲除小鼠无法杀死这种幼虫靶标。相比之下，接种疫苗的干扰素-γ基因敲除小鼠和C57BL/6小鼠表现出高水平的杀伤作用，其扩散室中的IL-4水平升高，嗜酸性粒细胞数量比IL-4基因敲除小鼠显著更多。虽然三组免疫小鼠中的干扰素-γ水平与对照小鼠相当，但即使在IL-4基因敲除小鼠中，IL-5水平也升高，这表明除IL-4外的其他细胞因子参与了其产生。在用辐照幼虫接种疫苗的小鼠中，对盘尾丝虫第三期幼虫的保护性免疫反应绝对需要IL-4。
