Koutsoumanis K, Tassou C C, Taoukis P S, Nychas G J
Agricultural University of Athens, Department of Food Science and Technology, Hellas, Greece.
J Appl Microbiol. 1998 Jun;84(6):981-7. doi: 10.1046/j.1365-2672.1998.00433.x.
The growth of Salmonella enteritidis in a brain heart infusion medium was monitored using the traditional viable count method and by conductance measurements using a Rabit impedance instrument. Growth curves (log10 cfu ml-1 vs time) at three different concentrations of oleuropein (0, 0.2 and 0.8%), pH values in the range of 5-8 and incubation temperatures from 22 to 42 degrees C were modelled using the Gompertz equation. A good correlation between the maximum growth rate from the viable count method and the maximum slope of the conductance curve from the impedance instrument was established. Based on this correlation, the maximum specific growth rate of Salm. enteritidis was modelled as a function of the oleuropein concentration, initial pH values and the incubation temperature with a quadratic equation, using a new, large dataset of growth measurements by conductance. The developed model was validated by statistical comparison of predicted growth rates with growth rates determined by the viable count method, within the limits of the antimicrobial, pH and temperature domain.
采用传统活菌计数法以及使用Rabit阻抗仪通过电导率测量来监测肠炎沙门氏菌在脑心浸液培养基中的生长情况。利用Gompertz方程对三种不同橄榄苦苷浓度（0%、0.2%和0.8%）、pH值范围为5 - 8以及培养温度为22至42摄氏度条件下的生长曲线（log10 cfu ml-1对时间）进行建模。在活菌计数法得到的最大生长速率与阻抗仪电导率曲线的最大斜率之间建立了良好的相关性。基于这种相关性，利用一个新的、通过电导率进行生长测量的大型数据集，用二次方程将肠炎沙门氏菌的最大比生长速率模拟为橄榄苦苷浓度、初始pH值和培养温度的函数。通过在抗菌、pH和温度范围内，将预测生长速率与活菌计数法测定的生长速率进行统计比较，对所建立的模型进行验证。
