注意：从鳗弧菌和副溶血性弧菌中克隆几丁质酶基因。

Note: molecular cloning of chitinase genes from Vibrio anguillarum and V. parahaemolyticus.

作者信息

Hirono I, Yamashita M, Aoki T

机构信息

Department of Biological Resources, Faculty of Agriculture, Miyazaki University, Japan.

出版信息

J Appl Microbiol. 1998 Jun;84(6):1175-8. doi: 10.1046/j.1365-2672.1998.00439.x.

DOI:10.1046/j.1365-2672.1998.00439.x

PMID:9717305

Abstract

Chitinase genes from Vibrio anguillarum KV9001 and V. parahaemolyticus ATCC17802 were cloned into Escherichia coli. Open reading frames of chitinase genes from V. anguillarum (vac) and V. parahaemolyticus (vpc) are 1755 bp and 1890 bp, respectively. The deduced amino acid sequences of these genes have 71.6% identity. There are two consensus sequence regions in the VAC and VPC proteins. The vac gene was highly prevalent in V. anguillarum, and the DNA probe of the vac gene hybridized to V. alginolyticus and Beneckea proteolytica DNA. The DNA probe of the upc gene hybridized to V. alginolyticus, V. harveyi and V. ordalii DNA.

摘要

将鳗弧菌KV9001和副溶血性弧菌ATCC17802的几丁质酶基因克隆到大肠杆菌中。鳗弧菌（vac）和副溶血性弧菌（vpc）几丁质酶基因的开放阅读框分别为1755 bp和1890 bp。这些基因推导的氨基酸序列具有71.6%的同一性。VAC和VPC蛋白中有两个共有序列区域。vac基因在鳗弧菌中高度流行，vac基因的DNA探针与溶藻弧菌和溶蛋白贝内克氏菌的DNA杂交。upc基因的DNA探针与溶藻弧菌、哈维氏弧菌和奥尔达利弧菌的DNA杂交。