Hume M E, Byrd J A, Stanker L H, Ziprin R L
USDA, Agricultural Research Service, College Station, TX, USA.
Lett Appl Microbiol. 1998 Jun;26(6):432-6. doi: 10.1046/j.1472-765x.1998.00371.x.
Day-of-hatch Leghorn chicks were treated by oral gavage with PREEMPT, a continuous-flow competitive exclusion culture containing broiler caecal bacteria, followed by an oral challenge with Listeria monocytogenes, to determine the effects of PREEMPT on L. monocytogenes caecal colonization. Increased (P < 0.001) concentrations of caecal propionic acid in control chicks compared with PREEMPT-treated chicks at 3 days of age were indicative of the establishment of the PREEMPT bacteria. Caeca from control chicks at 7 days after the oral challenge with L. monocytogenes contained mean 3.4 +/- 1.4 log cfu g-1 of caecal content, while caeca from PREEMPT-treated chicks contained no detectable Listeria. Enrichment for L. monocytogenes resulted in 100% of caeca from control chicks testing culture-positive for L. monocytogenes, while none of the caeca from PREEMPT-treated chicks were culture-positive. The results indicated that prophylactic treatment of newly hatched chicks with PREEMPT significantly reduced caecal colonization by L. monocytogenes.
刚孵化出的来航鸡雏鸡通过口服灌胃给予PREEMPT（一种含有肉鸡盲肠细菌的连续流动竞争性排斥培养物），随后口服单核细胞增生李斯特菌进行攻毒，以确定PREEMPT对单核细胞增生李斯特菌在盲肠定殖的影响。与3日龄时接受PREEMPT处理的雏鸡相比，对照雏鸡盲肠丙酸浓度升高（P < 0.001），这表明PREEMPT细菌已定植。在口服单核细胞增生李斯特菌攻毒7天后，对照雏鸡的盲肠中每克盲肠内容物平均含有3.4 +/- 1.4 log cfu，而接受PREEMPT处理的雏鸡盲肠中未检测到李斯特菌。对单核细胞增生李斯特菌进行富集培养后，对照雏鸡100%的盲肠培养检测呈单核细胞增生李斯特菌阳性，而接受PREEMPT处理的雏鸡盲肠均未培养阳性。结果表明，用PREEMPT对新孵化的雏鸡进行预防性处理可显著减少单核细胞增生李斯特菌在盲肠的定殖。
