Natori Y, Ou Z L, Yamamoto-Shuda Y, Natori Y
Division of Pathophysiology, Research Institute, International Medical Centre of Japan, Tokyo.
Clin Exp Immunol. 1998 Aug;113(2):265-8. doi: 10.1046/j.1365-2249.1998.00651.x.
Lymphotactin (LTN) is the sole member of C chemokine, the third subfamily of chemokines. LTN has been shown to be a chemoattractant specific for CD8+ cells and/or natural killer (NK) cells, and to be produced by CD8+ T cells, NK cells, and mast cells. However, there have been no reports describing its expression in clinical or experimental models of diseases so far. Since glomerular infiltration of CD8+ cells is prominent in an animal model of crescentic glomerulonephritis induced in WKY rats by an injection of anti-glomerular basement membrane antibody, we investigated the gene expression of LTN in this model. LTN mRNA was not detected in normal glomeruli but was detected at 0.5 h after the antibody injection, which detection preceded the infiltration of CD8+ cells. The expression of LTN mRNA peaked on day 3 and decreased thereafter. We next studied the expression of LTN mRNA in cultured glomerular and vascular cells, and found that glomerular mesangial and vascular endothelial cells could express LTN mRNA when stimulated with IL-1beta. These results indicate that the gene expression of LTN is enhanced in the animal model of glomerulonephritis and that intrinsic renal cells are the potential source of the gene expression of LTN in the kidney.
淋巴细胞趋化因子（LTN）是趋化因子第三个亚家族C趋化因子的唯一成员。LTN已被证明是一种对CD8 +细胞和/或自然杀伤（NK）细胞具有特异性的趋化因子，由CD8 + T细胞、NK细胞和肥大细胞产生。然而，迄今为止，尚无关于其在疾病临床或实验模型中表达的报道。由于在通过注射抗肾小球基底膜抗体诱导的WKY大鼠新月体性肾小球肾炎动物模型中，CD8 +细胞的肾小球浸润很明显，我们研究了该模型中LTN的基因表达。在正常肾小球中未检测到LTN mRNA，但在注射抗体后0.5小时检测到，该检测先于CD8 +细胞的浸润。LTN mRNA的表达在第3天达到峰值，此后下降。接下来，我们研究了培养的肾小球和血管细胞中LTN mRNA的表达，并发现肾小球系膜细胞和血管内皮细胞在受到IL-1β刺激时可以表达LTN mRNA。这些结果表明，在肾小球肾炎动物模型中LTN的基因表达增强，并且肾脏固有细胞是肾脏中LTN基因表达的潜在来源。
