Mercier J F, Slater G W
Department of Physics, University of Ottawa, Ontario, Canada.
Electrophoresis. 1998 Jul;19(10):1560-5. doi: 10.1002/elps.1150191006.
In this article, we extend our recently developed lattice model of gel electrophoresis to periodic three-dimensional gels made of either isolated obstacles or infinitely long fibers. Exact mobilities are calculated using a much improved numerical method that allows us to treat very large systems. A comparison of the exact mobilities and free available volumes indicates that the main assumption of the Ogston-Morris-Rodbard-Chrambach model (OMRCM), which postulates that the mobility (mu) of charged particles is directly related to the fractional gel volume available to them, is not valid. However, a study of the gel concentration and analyte size dependence of the zero-field mobility indicates that the OMRCM and the Ferguson plots can indeed be used to obtain useful, semi-quantitative information about the gel properties. A procedure to study more realistic three-dimensional gel systems is discussed.
在本文中，我们将最近开发的凝胶电泳晶格模型扩展到由孤立障碍物或无限长纤维构成的周期性三维凝胶。使用一种大大改进的数值方法计算精确迁移率，该方法使我们能够处理非常大的系统。精确迁移率与自由可用体积的比较表明，奥格斯顿 - 莫里斯 - 罗德巴德 - 克兰巴赫模型（OMRCM）的主要假设（该假设假定带电粒子的迁移率（μ）与它们可利用的凝胶体积分数直接相关）是无效的。然而，对零场迁移率的凝胶浓度和分析物大小依赖性的研究表明，OMRCM和弗格森图确实可用于获取有关凝胶性质的有用半定量信息。本文讨论了研究更现实的三维凝胶系统的方法。
