Cann J R
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA.
Electrophoresis. 1998 Jul;19(10):1577-85. doi: 10.1002/elps.1150191010.
The theory of mass transport coupled to reversible protein interactions forms the basis for computer simulation of the isoelectric focusing behavior of several model systems. These include pH-dependent conformational transition, carrier ampholyte-induced interactions and protein-ligand interactions. The computational results compare favorably with experimental observations. In addition, a method is formulated for an isoelectric focusing procedure which enables determination of intrinsic ligand-binding constants for statistical binding of a charged ligand, binding to heterogeneous sites, and cooperative binding.
与可逆蛋白质相互作用耦合的质量传输理论构成了几个模型系统等电聚焦行为计算机模拟的基础。这些包括pH依赖性构象转变、载体两性电解质诱导的相互作用和蛋白质-配体相互作用。计算结果与实验观察结果相比非常有利。此外，还制定了一种等电聚焦程序的方法，该方法能够确定带电配体的统计结合、与异质位点的结合以及协同结合的内在配体结合常数。
