Stoyanov A V, Righetti P G
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Electrophoresis. 1998 Jul;19(10):1596-600. doi: 10.1002/elps.1150191013.
The equation of balance between electrophoretic and diffusional mass flows in the steady state of isoelectric focusing is analyzed. To create the pH gradient, a model system composed of only one Immobiline is used. The solution is found for the case of a small sample concentration, but without assumptions about linear "focusing force" and constant (linear) conductivity profiles. The effect of sample concentration on the final concentration profile is also evaluated. At high sample concentrations, it is demonstrated that the steady-state distribution is essentially non-Gaussian and results in a considerably lower concentration maximum as compared with low sample levels.
分析了等电聚焦稳态下电泳和扩散质量流之间的平衡方程。为了创建pH梯度，使用了仅由一种固定化电解质组成的模型系统。找到了小样品浓度情况下的解，但没有对线性“聚焦力”和恒定（线性）电导率分布做假设。还评估了样品浓度对最终浓度分布的影响。在高样品浓度下，结果表明稳态分布本质上是非高斯的，与低样品水平相比，其浓度最大值要低得多。
