Ahne W, Bearzotti M, Bremont M, Essbauer S
Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich, München, Germany.
Zentralbl Veterinarmed B. 1998 Aug;45(6):373-83. doi: 10.1111/j.1439-0450.1998.tb00806.x.
Iridovirus-like agents isolated from systemic infected fish (Silurus glanis, SFIR; Ictalurus melas, CFIR I, CFIR II, CFIR III) and from frogs (Rana esculenta, REIR) in Europe, Epizootic Haematopoietic Necrosis Virus (EHNV) isolated in Australia from redfin perch (Perca fluviatilis), and Frog Virus 3 (FV 3) isolated from frogs (Rana pipiens) in the USA were investigated by electron microscopy, polypeptide composition, immunofluorescence, restriction endonuclease digestion, Southern-blot hybridization and polymerase chain reaction (PCR) amplification. All virus isolates proved to be similar in morphology and in size and reacted with EHNV polyclonal antiserum in the immunofluorescence. Whilst DNA restriction profiles of the European piscine isolates cleaved by BamH I were similar, they differed clearly from those of EHNV, REIR and FV 3. Southern-blot analysis of viral BamH I digested DNA using an EHNV DNA probe revealed cross-hybridization with DNA of the investigated iridoviruses. Using a set of primers designed for an open reading frame of the EHNV genome, PCR products of about 250 bp were obtained with the DNA of systemic piscine and amphibian iridoviruses. The data suggest that the systemic piscine and amphibian iridoviruses should be regarded as members of the the genus Ranavirus within the family Iridoviridae.
对从欧洲系统性感染的鱼类（六须鲶，SFIR；黑叉尾鮰，CFIR I、CFIR II、CFIR III）和青蛙（食用蛙，REIR）中分离出的虹彩病毒样病原体、从澳大利亚红鳍鲈（河鲈）中分离出的流行性造血器官坏死病毒（EHNV）以及从美国青蛙（豹蛙）中分离出的蛙病毒3（FV 3）进行了电子显微镜检查、多肽组成分析、免疫荧光检测、限制性内切酶消化、Southern印迹杂交和聚合酶链反应（PCR）扩增。所有病毒分离株在形态和大小上均相似，且在免疫荧光检测中与EHNV多克隆抗血清发生反应。虽然经BamH I酶切的欧洲鱼类分离株的DNA限制性图谱相似，但它们与EHNV、REIR和FV 3的图谱明显不同。使用EHNV DNA探针对病毒BamH I酶切DNA进行Southern印迹分析，结果显示与所研究的虹彩病毒的DNA存在交叉杂交。使用针对EHNV基因组一个开放阅读框设计的一组引物，从系统性感染的鱼类和两栖类虹彩病毒的DNA中获得了约250 bp的PCR产物。数据表明，系统性感染的鱼类和两栖类虹彩病毒应被视为虹彩病毒科蛙病毒属的成员。
