光学生物成像中的非侵入性图像采集与高级处理

Non-invasive image acquisition and advanced processing in optical bioimaging.

作者信息

Farkas D L, Du C, Fisher G W, Lau C, Niu W, Wachman E S, Levenson R M

机构信息

Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA. farkas+@cmu.edu

出版信息

Comput Med Imaging Graph. 1998 Mar-Apr;22(2):89-102. doi: 10.1016/s0895-6111(98)00011-1.

DOI:10.1016/s0895-6111(98)00011-1

PMID:9719850

Abstract

Light is a most versatile tool for investigating biological systems and phenomena; the range, non-destructiveness, spatial discrimination and speed of optical imaging are all important for investigating structure and function at the cellular, tissue or even whole organism level. In live biological imaging, where the technological requirements are heightened, other features of light, such as coherence and wavelength, are used to generate the additional contrast and resolution needed. We report here recent improvements in our ability to image biological specimens optically, focusing on (a) spectral resolution and the related image processing issues, and (b) tomographic three-dimensional fluorescence imaging in vivo.

摘要

光对于研究生物系统和现象而言是一种极为通用的工具；光学成像的范围、非破坏性、空间分辨能力和速度对于在细胞、组织乃至整个生物体层面研究结构和功能都非常重要。在对技术要求更高的活体细胞成像中，光的其他特性，如相干性和波长，被用于产生所需的额外对比度和分辨率。我们在此报告近期在光学成像生物样本能力方面取得的进展，重点关注（a）光谱分辨率及相关图像处理问题，以及（b）体内断层三维荧光成像。