[枯草芽孢杆菌中ribR基因的克隆与生化鉴定]
[Cloning and biochemical identification of the ribR gene in Bacillus subtilis].
作者信息
Solov'eva I M, Kreneva R A, Polanuer B M, Kozlov Iu I, Perumov D A
机构信息
Department of Molecular and Radiation Biophysics, Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast', Russia.
出版信息
Genetika. 1998 Jun;34(6):839-42.
PMID:9719928
Abstract
The PCR copy of the ribR gene of Bacillus subtilis was subcloned in Escherichia coli cells under the control of the phage T7 inducible promoter. The polypeptide of 26 kDa corresponding to the 690-bp gene is the product of the ribR gene. The protein encoded by the ribR gene is flavokinase, and the riboflavin-reduced form is the substrate for it.
摘要
枯草芽孢杆菌ribR基因的PCR拷贝在噬菌体T7诱导型启动子的控制下亚克隆到大肠杆菌细胞中。与690 bp基因相对应的26 kDa多肽是ribR基因的产物。ribR基因编码的蛋白质是黄素激酶，核黄素还原形式是其底物。
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