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[枯草芽孢杆菌中ribR基因活性调控机制的研究]

[Study of the mechanism for regulating ribR gene activity in Bacillus subtilis].

作者信息

Solov'eva I M, Kreneva R A, Erraĭs L L, Mironov A S, Perumov D A

出版信息

Genetika. 2004 May;40(5):716-20.

Abstract

Analysis of the phenotypic manifestation of inactivation of several genes from the ytlI-ytnM operon containing the ribR gene and results of Northern hybridization showed that the ribR gene does not have the self promoter and is transcribed from the main promoter of the ytlI-ytnM operon. Two sites of single nucleotide substitutions leading to derepresson of ribR gene were identified between the putative main promoter and transcription start of the ytlI-ytnM operon regulatory region.

摘要

对含有ribR基因的ytlI - ytnM操纵子中几个基因失活的表型表现进行分析,并结合Northern杂交结果表明,ribR基因没有自身启动子,而是从ytlI - ytnM操纵子的主要启动子转录而来。在ytlI - ytnM操纵子调控区假定的主要启动子和转录起始点之间,鉴定出两个导致ribR基因去阻遏的单核苷酸取代位点。

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