Zeiss C J, Trepanier L A, Aguirre G D, Ray K
James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Anim Genet. 1998 Jun;29(3):224-7. doi: 10.1046/j.1365-2052.1998.00329.x.
The presence of a CA repeat within the 3'-untranslated region (UTR) of the dystrophin gene has been reported previously in several species. Because microsatellites showing high cross-species homology can be conveniently used as markers in those species for which detailed linkage maps have not yet been developed, we evaluated whether the CA repeat could be amplified from a wide variety of mammalian species. Using a single pair of canine-specific oligonucleotide primers, we successfully amplified the 3'-UTR from 18 different carnivore and six additional species (human, chimpanzee, goat, cow, rabbit and mouse) and show conservation of the CA repeat in the dystrophin gene from a wide range of evolutionarily diverse mammalian species.
此前已有报道称，在多个物种的肌营养不良蛋白基因的3'-非翻译区（UTR）中存在CA重复序列。由于显示出高跨物种同源性的微卫星可以方便地用作那些尚未开发详细连锁图谱的物种的标记，因此我们评估了是否可以从多种哺乳动物物种中扩增出CA重复序列。使用一对犬特异性寡核苷酸引物，我们成功地从18种不同的食肉动物以及另外6种物种（人类、黑猩猩、山羊、牛、兔子和小鼠）中扩增出了3'-UTR，并表明在广泛进化多样的哺乳动物物种的肌营养不良蛋白基因中，CA重复序列具有保守性。
