Ikeda K, Sun F, Tanaka K, Tokumaru S, Kojo S
Department of Food Science and Nutrition, Nara Women's University, Japan.
Biosci Biotechnol Biochem. 1998 Jul;62(7):1438-9. doi: 10.1271/bbb.62.1438.
Two hours after an intraperitoneal injection of ferric nitrilotriacetate to rats at a dose of 15 mg of Fe/kg of body weight, the level of lipid hydroperoxides, which was determined by a specific method involving chemical conversion into 1-naphthyldiphenylphosphine oxide and HPLC, had increased significantly in the liver (from 190.1 +/- 58.8 of the control to 467.1 +/- 175.8 pmol/mg of protein) and kidney (from 181.8 +/- 52.3 of the control to 405.9 +/- 22.7 pmol/mg of protein). These results demonstrate that oxidative stress was transiently increased by an iron overload in these tissues.
以15毫克铁/千克体重的剂量给大鼠腹腔注射次氮基三乙酸铁两小时后，通过将其化学转化为1-萘基二苯基氧化膦并进行高效液相色谱分析的特定方法测定的脂质氢过氧化物水平，在肝脏（从对照的190.1±58.8升至467.1±175.8皮摩尔/毫克蛋白质）和肾脏（从对照的181.8±52.3升至405.9±22.7皮摩尔/毫克蛋白质）中显著升高。这些结果表明，这些组织中的铁过载会使氧化应激短暂增加。
