Yamakawa N, Tanaka T, Kezuka T, Usui M
Department of Ophthalmology, Tokyo Medical College, Japan.
Nippon Ganka Gakkai Zasshi. 1998 Jul;102(7):411-6.
Heat-shock protein 60 derived from Yersinia enterocolitica (Yersinia HSP 60) and bovine retinal HSP (Retina HSP 60) were previously identified by immunological cross-reaction and a high degree of common antigenicity, and a specific antibody against both proteins was detected in the sera of uveitis patients. We report here an attempt to isolate and purify Retina HSP 60 and Yersinia HSP 60. Both Retina HSP 60 and Yersinia HSP 60 showed an enriched content of glycine of approximately 60 to 80%. Lewis rats were inculated with 50 or 100 micrograms of purified Retina or Yersinia HSP 60 emulsified in complete Freund's adjuvant. In 50 to 60% of those inoculated with Retina HSP 60, uveoretinitis was observed about 13 days after inoculation, with massive infiltration of lymphocytes and polymorphonuclear neutrophils in the iris, ciliary body, and retinal tissue. Rats inoculated with Yersinia HSP 60 did not develop ocular inflammation. Lymphocyte proliferation assay was performed to investigate cellular immunoresponses in the rats that developed ocular inflammation after immunization with Retina HSP 60. The results showed significantly higher response to the Retina and Yersinia HSP 60 than to either S-antigen or interphotoreceptor retinoid-binding protein (IRBP), which are known to induce ocular inflammation. Cross-reaction between Retina and Yersinia HSP 60 is suggested. This study suggests that the HSP 60 molecules may be involved in the pathogenesis of intraocular inflammation.
先前通过免疫交叉反应和高度的共同抗原性鉴定出了来自小肠结肠炎耶尔森菌的热休克蛋白60（耶尔森菌热休克蛋白60）和牛视网膜热休克蛋白（视网膜热休克蛋白60），并且在葡萄膜炎患者的血清中检测到了针对这两种蛋白的特异性抗体。我们在此报告了一项分离和纯化视网膜热休克蛋白60和耶尔森菌热休克蛋白60的尝试。视网膜热休克蛋白60和耶尔森菌热休克蛋白60均显示甘氨酸含量丰富，约为60%至80%。将50或100微克纯化的视网膜或耶尔森菌热休克蛋白60乳化于完全弗氏佐剂中，接种给Lewis大鼠。在接种视网膜热休克蛋白60的大鼠中，约50%至60%在接种后约13天出现葡萄膜视网膜炎，虹膜、睫状体和视网膜组织中有大量淋巴细胞和多形核中性粒细胞浸润。接种耶尔森菌热休克蛋白60的大鼠未发生眼部炎症。进行淋巴细胞增殖试验以研究在用视网膜热休克蛋白60免疫后发生眼部炎症的大鼠中的细胞免疫反应。结果显示，与已知可诱导眼部炎症的S抗原或光感受器间类视黄醇结合蛋白（IRBP）相比，对视网膜和耶尔森菌热休克蛋白60的反应明显更高。提示视网膜热休克蛋白60和耶尔森菌热休克蛋白60之间存在交叉反应。本研究表明热休克蛋白60分子可能参与眼内炎症的发病机制。
