Fogt F, Vortmeyer A O, Poremba C, Minda M, Harris C A, Tomaszewski J E
Department of Pathology, University of Pennsylvania, Philadelphia 19104, USA.
Mod Pathol. 1998 Aug;11(8):716-20.
Archival paraffin-embedded tissue from 5 normal adrenal glands (including 1 from a fetus of 28 weeks' gestation), 6 cases of adrenal cortical hyperplasia, 9 cortical adenomas, 14 cortical carcinomas, and 11 pheochromocytomas were immunostained with monoclonal antibody against bcl-2. Ultrastructural localization of bcl-2 protein was also performed on selected cases. Positive immunostaining for bcl-2 was seen in all of the layers of the normal adrenal cortex, with different staining characteristics. bcl-2 expression was never observed in the normal adrenal medulla. Electron microscopic studies revealed bcl-2 to be localized predominantly to mitochondria, with a small number of labels along the nuclear envelope. Analysis of adrenal neoplasms showed expression of bcl-2 in cortical tumors, but only one positive case in pheochromocytomas. Restriction of bcl-2 expression to adrenal cortex-derived tissue versus adrenal medulla-derived tissue might prove to be helpful for the differential diagnosis between cortical and medullary tumors.
用抗bcl-2单克隆抗体对5例正常肾上腺（包括1例孕28周胎儿的肾上腺）、6例肾上腺皮质增生、9例皮质腺瘤、14例皮质癌和11例嗜铬细胞瘤的存档石蜡包埋组织进行免疫染色。还对部分病例进行了bcl-2蛋白的超微结构定位。在正常肾上腺皮质的所有层中均可见bcl-2的阳性免疫染色，且具有不同的染色特征。在正常肾上腺髓质中从未观察到bcl-2表达。电子显微镜研究显示bcl-2主要定位于线粒体，核膜上有少量标记。肾上腺肿瘤分析显示bcl-2在皮质肿瘤中表达，但嗜铬细胞瘤中只有1例阳性。bcl-2表达局限于肾上腺皮质来源组织与肾上腺髓质来源组织，这可能有助于皮质和髓质肿瘤的鉴别诊断。
