Tei S, Farnesi R M, Santarella B, Secca T, Vagnetti D
Institute of Comparative Anatomy, University of Perugia, Italy.
J Submicrosc Cytol Pathol. 1998 Jul;30(3):355-63.
The ultracytochemical localization of particulate guanylate cyclase (GC) has been studied in Rana esculenta choroid plexus after activation with rat atrial natriuretic peptide (ANP), porcine brain natriuretic peptide (BNP) and porcine C-type natriuretic peptide (CNP). This study shows that the three peptides are activators of GC in the choroid plexus as demonstrated by the presence of reaction products at the level of the epithelium and sinusoids. In the apical zone of the epithelial cells predominantly BNP seems to activate GC, while ANP and CNP activate GC mainly at the level of the lateral surfaces. Moreover, ANP stimulates the enzyme along the basal membrane of the epithelial cells as well as the membranes of the endothelium of the sinusoids. In the presence of CNP, enzyme stimulation can also be found at the level of the endocellular membranes. These results confirm that the choroid plexus is an organ with receptors for the natriuretic peptides which are involved in the processes of osmoregulation and the control of cerebrospinal fluid production.
在用大鼠心房利钠肽（ANP）、猪脑利钠肽（BNP）和猪C型利钠肽（CNP）激活后，对食用蛙脉络丛中颗粒型鸟苷酸环化酶（GC）进行了超细胞化学定位研究。本研究表明，这三种肽是脉络丛中GC的激活剂，上皮和血窦水平存在反应产物证明了这一点。在上皮细胞的顶端区域，主要是BNP似乎激活了GC，而ANP和CNP主要在侧面激活GC。此外，ANP沿着上皮细胞的基底膜以及血窦内皮细胞膜刺激该酶。在CNP存在的情况下，在内细胞膜水平也能发现酶的刺激。这些结果证实，脉络丛是一个具有利钠肽受体的器官，这些受体参与渗透调节和脑脊液生成的控制过程。
