Aliev G, Ragazzi E, Cirillo R, Bevilacqua C, Mironov A, Prosdocimi M, Paro M
Lerner Research Institute, Cleveland Clinic Foundation, OH, USA.
J Submicrosc Cytol Pathol. 1998 Jul;30(3):417-23.
In the present study we developed an experimental model, resembling human atherosclerosis, by removing the endothelial layer in Watanabe heritable hyperlipidemic (WHHL) rabbits (10 months old) by application of cryodamage on the external surface of arterial vessels. In age-matched New Zealand white (NZW) rabbits, used as control, after two months following cryodamage, carotid artery and infrarenal segments of abdominal aorta did not show any particular change in the ultrastructure of vessel wall. In WHHL rabbits, two months after cryodamage, atherosclerotic lesions (fatty streaks and fibrous plaques) were observed in both arteries. Many lipid-laden endothelial cells, subendothelial foam cells and smooth muscle cells were found in cryodamaged areas. In some areas, the cap of plaques appeared to be thinned and ruptured. Increased number of collagen and elastic fibrils was also observed in atherosclerotic regions. We conclude that this simple technique on WHHL rabbits provides a model of atherosclerosis with a high degree of morphological similarity between the artificially-induced plaque and human atherosclerotic plaque.
在本研究中，我们通过对10月龄的渡边遗传性高脂血症（WHHL）兔的动脉血管外表面进行冷冻损伤来去除内皮，从而建立了一个类似于人类动脉粥样硬化的实验模型。在作为对照的年龄匹配的新西兰白兔（NZW）中，冷冻损伤两个月后，颈动脉和腹主动脉肾下段的血管壁超微结构未显示任何特殊变化。在WHHL兔中，冷冻损伤两个月后，在两条动脉中均观察到动脉粥样硬化病变（脂肪条纹和纤维斑块）。在冷冻损伤区域发现了许多充满脂质的内皮细胞、内皮下泡沫细胞和平滑肌细胞。在一些区域，斑块的帽似乎变薄并破裂。在动脉粥样硬化区域还观察到胶原纤维和弹性纤维数量增加。我们得出结论，这种在WHHL兔上的简单技术提供了一种动脉粥样硬化模型，人工诱导的斑块与人类动脉粥样硬化斑块在形态上具有高度相似性。
