Garssen J, Buckley T L, Van Loveren H
Laboratory for Pathology and Immunobiology, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
Photochem Photobiol. 1998 Aug;68(2):205-10.
The aim of this study was to investigate the possible role of sensory nerves in UV light-induced systemic immunomodulation. Contact hypersensitivity to the low molecular weight compound picrylchloride was used as a model for cellular immunity that can be suppressed by low (i.e. suberythemal) doses of UV light even after exposure at a distant locus (i.e. systemic immunosuppression). In sensory nerve-depleted mice, achieved by two subcutaneous injections with the neurotoxin capsaicin before the age of 4 weeks, UV light exposure failed to inhibit contact hypersensitivity responses to picrylchloride. This indicates that sensory nerves are at least partially involved in the induction of systemic immunosuppression by UV light. In order to analyze whether sensory neuropeptides, such as calcitonin gene-related peptide (CGRP) and tachykinins, are involved in UV light-induced systemic immunosuppression, mice were pretreated with selective antagonists prior to each UV light exposure. These experiments indicated that CGRP but not the tachykinins plays a crucial role in the UV light-induced systemic immunosuppression.
本研究的目的是调查感觉神经在紫外线诱导的全身免疫调节中可能发挥的作用。对低分子量化合物苦味酸氯的接触性超敏反应被用作细胞免疫的模型，低剂量（即亚红斑剂量）的紫外线即使在远处部位暴露后（即全身免疫抑制）也能抑制这种反应。在4周龄前通过两次皮下注射神经毒素辣椒素使小鼠感觉神经耗竭，紫外线照射未能抑制对苦味酸氯的接触性超敏反应。这表明感觉神经至少部分参与了紫外线诱导的全身免疫抑制的诱导过程。为了分析感觉神经肽，如降钙素基因相关肽（CGRP）和速激肽，是否参与紫外线诱导的全身免疫抑制，在每次紫外线照射前用选择性拮抗剂对小鼠进行预处理。这些实验表明，CGRP而非速激肽在紫外线诱导的全身免疫抑制中起关键作用。
