Betz U A, Müller W
Institute for Genetics, University of Cologne, Germany.
Int Immunol. 1998 Aug;10(8):1175-84. doi: 10.1093/intimm/10.8.1175.
The functional receptor for the inflammatory cytokine IL-6 is composed of the ligand binding IL-6 receptor alpha chain (IL-6R alpha) and the signal transducing chain gp130, which is a shared component of multiple cytokine receptors. We analyzed the surface expression of gp130 and IL-6R alpha in thymocytes and peripheral T cells. While all thymocytes expressed gp130 throughout thymic maturation, they gained expression of IL-6R alpha at the CD4 or CD8 single-positive stage. Approximately 10-30% of the CD4-CD8+ and 40-50% of the CD4+CD8- thymocytes expressed IL-6R alpha. Within the CD4+CD8- population, the IL-6R alpha- subpopulation was cortisone sensitive, appeared immature according to the cell surface markers expressed and failed to proliferate after TCR cross-linking. Peripheral T cells were predominantly gp130+ and IL-6R alpha+, but down-regulated gp130 and IL-6R alpha expression upon TCR engagement in vitro and in vivo. Peripheral gp130low/-IL-6R alphalow/- T cells expressed surface markers characteristic of memory T cells. We show that gp130 and IL-6R alpha are expressed in a regulated manner in T cells, depending on the developmental and functional stage.
炎性细胞因子白细胞介素-6（IL-6）的功能性受体由结合配体的IL-6受体α链（IL-6Rα）和信号转导链gp130组成，gp130是多种细胞因子受体的共享成分。我们分析了胸腺细胞和外周T细胞中gp130和IL-6Rα的表面表达情况。虽然所有胸腺细胞在整个胸腺成熟过程中都表达gp130，但它们在CD4或CD8单阳性阶段获得了IL-6Rα的表达。大约10%-30%的CD4-CD8+胸腺细胞和40%-50%的CD4+CD8-胸腺细胞表达IL-6Rα。在CD4+CD8-群体中，IL-6Rα阴性亚群对可的松敏感，根据所表达的细胞表面标志物显示出不成熟，并且在TCR交联后不能增殖。外周T细胞主要是gp130+和IL-6Rα+，但在体外和体内TCR参与后会下调gp130和IL-6Rα的表达。外周gp130低表达/缺失-IL-6Rα低表达/缺失的T细胞表达记忆T细胞特有的表面标志物。我们表明，gp130和IL-6Rα在T细胞中以一种受调控的方式表达，这取决于发育和功能阶段。
