准分子激光深层环形角膜切除术后基质内迁移上皮细胞表达的明胶酶B的免疫共聚焦定位
Immunoconfocal localization of gelatinase B expressed by migrating intrastromal epithelial cells after deep annular excimer keratectomy.
作者信息
Maeda M, Vanlandingham B D, Ye H, Lu P C, Azar D T
机构信息
Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, and Department of Ophthalmology, Harvard Medical School, Boston, USA.
出版信息
Curr Eye Res. 1998 Aug;17(8):836-43.
PURPOSE
Intrastromal epithelial migration occurs following deep excimer annular keratectomy. Our purpose is to determine the localization of gelatinase B expression and its relationship to migrating intrastromal epithelial cells following deep annular excimer keratectomy.
METHODS
Rabbit corneas were treated with deep annular ablation and harvested 3, 5, 7 and 14 days following surgery. Histological examination was performed. Confocal microscopy was used to determine the time and the pattern of gelatinase B expression. This was compared to AE-5, vimentin, and alpha-smooth muscle actin immunolocalization.
RESULTS
Histologic examination revealed islands of epithelial cells within the corneal stroma after deep annular excimer ablation, whereas eyes treated with superficial annular keratectomy did not show intrastromal epithelial migration. Immunoconfocal microscopy using monoclonal anti-matrix metalloproteinase-9 (MMP-9) antibody demonstrated the presence of gelatinase B at the outer borders of the intrastromal epithelial islands following deep annular excimer keratectomy.
CONCLUSION
Gelatinase B may be involved in the process of intrastromal epithelial migration following deep annular excimer keratectomy.
目的
准分子激光深层环形角膜切除术之后会发生基质内上皮细胞迁移。我们的目的是确定明胶酶B表达的定位及其与准分子激光深层环形角膜切除术后基质内迁移上皮细胞的关系。
方法
对兔角膜进行深层环形消融，并在术后3、5、7和14天采集样本。进行组织学检查。使用共聚焦显微镜确定明胶酶B表达的时间和模式。将其与AE-5、波形蛋白和α-平滑肌肌动蛋白免疫定位进行比较。
结果
组织学检查显示，准分子激光深层环形消融术后角膜基质内出现上皮细胞岛，而浅层环形角膜切除术治疗的眼未出现基质内上皮细胞迁移。使用抗基质金属蛋白酶-9（MMP-9）单克隆抗体的免疫共聚焦显微镜检查显示，准分子激光深层环形角膜切除术后，基质内上皮岛的外边界存在明胶酶B。
结论
明胶酶B可能参与准分子激光深层环形角膜切除术后基质内上皮细胞迁移过程。
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