Rogers B B, Sommerauer J, Quan A, Timmons C F, Dawson D B, Scheuermann R H, Krisher K, Atkins C
Department of Pathology, Children's Medical Center, 1935 Motor Street, Dallas, TX 75235, USA.
Pediatr Dev Pathol. 1998 Nov-Dec;1(6):480-6. doi: 10.1007/s100249900066.
To assess whether the semiquantitative peripheral blood Epstein-Barr virus (EBV) polymerase chain reaction (PCR) test correlates with post-transplant lymphoproliferative disorder (LPD), we compiled the results of the test done over a 3-year period ending July 1997. Six hundred seventy-six tests were done on 185 patients. Four hundred-thirty tests (63%) were negative, 167 (25%) were weak positive, 67 (10%) were moderate positive, and 12 (2%) were strong positive. Twelve of the patients developed a lymphoproliferative disorder (LPD) during this time. The EBV PCR tests proximate to the diagnosis of LPD in the 12 patients with EBV-positive LPD were 6 strong positive, 5 moderate positive, 1 weak positive. No patient with LPD had a negative result at diagnosis. Stated another way, 6/12 (50%) of strong-positive PCR tests, 5/67 (7%) moderate-positive tests, and 1/167 (.6%) of weak-positive tests correlated with LPD. Serologic evaluation for EBV done on 7 patients at the time of LPD showed low serologic responses in 5 of the 7 patients. The EBV PCR temporally associated with the serology indicated moderate to large viral burdens. In each patient evaluated serially, the EBV PCR test rose before the diagnosis of LPD and fell with treatment for the disorder. In conclusion, the EBV PCR test may be used as an adjunct to the diagnosis of patients with LPD and may be used to monitor response to therapy for the disorder.
为评估半定量外周血爱泼斯坦-巴尔病毒（EBV）聚合酶链反应（PCR）检测结果与移植后淋巴细胞增生性疾病（LPD）是否相关，我们汇总了截至1997年7月的3年期间所做检测的结果。对185例患者进行了676次检测。430次检测（63%）为阴性，167次（25%）为弱阳性，67次（10%）为中度阳性，12次（2%）为强阳性。在此期间，12例患者发生了淋巴细胞增生性疾病（LPD）。12例EBV阳性LPD患者在接近LPD诊断时的EBV PCR检测结果为6次强阳性、5次中度阳性、1次弱阳性。LPD患者在诊断时均无阴性结果。换言之，强阳性PCR检测结果中有6/12（50%）、中度阳性检测结果中有5/67（7%）、弱阳性检测结果中有1/167（0.6%）与LPD相关。7例LPD患者在LPD发生时进行的EBV血清学评估显示，7例患者中有5例血清学反应较低。与血清学在时间上相关的EBV PCR显示病毒载量为中度至大量。在每例接受连续评估的患者中，EBV PCR检测结果在LPD诊断前升高，在疾病治疗后下降。总之，EBV PCR检测可作为LPD患者诊断的辅助手段，并可用于监测该疾病的治疗反应。
