PC4与解链DNA的相互作用会抑制转录。
Interaction of PC4 with melted DNA inhibits transcription.
作者信息
Werten S, Stelzer G, Goppelt A, Langen F M, Gros P, Timmers H T, Van der Vliet P C, Meisterernst M
机构信息
Laboratorium voor Fysiologische Chemie, Utrecht University, Stratenum Universiteitsweg 100, Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands.
出版信息
EMBO J. 1998 Sep 1;17(17):5103-11. doi: 10.1093/emboj/17.17.5103.
Abstract
PC4 is a nuclear DNA-binding protein that stimulates activator-dependent class II gene transcription in vitro. Recent biochemical and X-ray analyses have revealed a unique structure within the C-terminal domain of PC4 that binds tightly to unpaired double-stranded (ds)DNA. The cellular function of this evolutionarily conserved dimeric DNA-binding fold is unknown. Here we demonstrate that PC4 represses transcription through this motif. Interaction with melted promoters is not required for activator-dependent transcription in vitro. The inhibitory activity is attenuated on bona fide promoters by (i) transcription factor TFIIH and (ii) phosphorylation of PC4. PC4 remains a potent inhibitor of transcription in regions containing unpaired ds DNA, in single-stranded DNA that can fold into two antiparallel strands, and on DNA ends. Our observations are consistent with a novel inhibitory function of PC4.
摘要
PC4是一种核DNA结合蛋白，在体外可刺激依赖激活因子的II类基因转录。最近的生化分析和X射线分析揭示了PC4 C端结构域内的一种独特结构，该结构与未配对的双链（ds）DNA紧密结合。这种进化上保守的二聚体DNA结合折叠的细胞功能尚不清楚。在此，我们证明PC4通过该基序抑制转录。体外依赖激活因子的转录不需要与解链的启动子相互作用。在真正的启动子上，（i）转录因子TFIIH和（ii）PC4的磷酸化可减弱其抑制活性。在含有未配对dsDNA的区域、可折叠成两条反平行链的单链DNA以及DNA末端，PC4仍然是一种有效的转录抑制剂。我们的观察结果与PC4的一种新的抑制功能一致。
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