MarA中一种新型的DNA结合基序：AraC家族转录激活因子的首个结构。
A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator.
作者信息
Rhee S, Martin R G, Rosner J L, Davies D R
机构信息
Laboratory of Molecular Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0560, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10413-8. doi: 10.1073/pnas.95.18.10413.
Abstract
A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.
摘要
本文描述了原核生物转录激活因子AraC家族成员MarA与其同源DNA结合位点形成的晶体结构。MarA由两个相似的亚结构域组成，每个亚结构域都包含一个螺旋-转角-螺旋DNA结合基序。基序中的两个识别螺旋插入到DNA同一面上相邻的大沟片段中，但仅相隔27埃，从而使DNA弯曲约35度。识别螺旋与DNA大沟之间广泛的相互作用提供了序列特异性。
相似文献
1
A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator.MarA中一种新型的DNA结合基序：AraC家族转录激活因子的首个结构。
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10413-8. doi: 10.1073/pnas.95.18.10413.
2
Crystal structure of the Escherichia coli Rob transcription factor in complex with DNA.大肠杆菌Rob转录因子与DNA复合物的晶体结构。
Nat Struct Biol. 2000 May;7(5):424-30. doi: 10.1038/75213.
3
Structural homology between MarA of the AraC family of transcriptional activators and the integrase family of site-specific recombinases.转录激活因子AraC家族的MarA与位点特异性重组酶整合酶家族之间的结构同源性。
Mol Microbiol. 2000 Mar;35(6):1582-3. doi: 10.1046/j.1365-2958.2000.01803.x.
4
A comprehensive alanine scanning mutagenesis of the Escherichia coli transcriptional activator SoxS: identifying amino acids important for DNA binding and transcription activation.大肠杆菌转录激活因子SoxS的全面丙氨酸扫描诱变：鉴定对DNA结合和转录激活重要的氨基酸
J Mol Biol. 2002 Sep 13;322(2):237-57. doi: 10.1016/s0022-2836(02)00782-9.
5
Probing the Escherichia coli transcriptional activator MarA using alanine-scanning mutagenesis: residues important for DNA binding and activation.利用丙氨酸扫描诱变探究大肠杆菌转录激活因子MarA：对DNA结合和激活重要的残基
J Mol Biol. 2000 Jun 23;299(5):1245-55. doi: 10.1006/jmbi.2000.3827.
6
Mutational analysis of the highly conserved C-terminal residues of the XylS protein, a member of the AraC family of transcriptional regulators.对转录调节因子AraC家族成员XylS蛋白高度保守的C末端残基进行突变分析。
FEBS Lett. 2000 Jul 7;476(3):312-7. doi: 10.1016/s0014-5793(00)01749-x.
7
Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices.与DNA结合的LacI成员PurR的晶体结构：α螺旋与小沟结合
Science. 1994 Nov 4;266(5186):763-70. doi: 10.1126/science.7973627.
8
Amino acid-DNA contacts by RhaS: an AraC family transcription activator.由RhaS形成的氨基酸与DNA的相互作用：一种AraC家族转录激活因子。
J Bacteriol. 1999 Sep;181(17):5185-92. doi: 10.1128/JB.181.17.5185-5192.1999.
9
Structure and function of the arginine repressor-operator complex from Bacillus subtilis.枯草芽孢杆菌精氨酸阻遏物-操纵基因复合物的结构与功能
J Mol Biol. 2008 May 30;379(2):284-98. doi: 10.1016/j.jmb.2008.03.007. Epub 2008 Mar 12.
10
The XylS/AraC family of regulators.XylS/AraC 调节因子家族。
Nucleic Acids Res. 1993 Feb 25;21(4):807-10. doi: 10.1093/nar/21.4.807.
引用本文的文献
1
Coordination of cell envelope biology by Escherichia coli MarA protein potentiates intrinsic antibiotic resistance.大肠杆菌MarA蛋白对细胞包膜生物学的协调作用增强了内在抗生素抗性。
PLoS Genet. 2025 May 5;21(5):e1011639. doi: 10.1371/journal.pgen.1011639. eCollection 2025 May.
2
Discovery of synthetic small molecules targeting the central regulator of pathogenicity.靶向致病性中心调节因子的合成小分子的发现
Sci Adv. 2025 Apr 11;11(15):eadr5235. doi: 10.1126/sciadv.adr5235.
3
Targeting MarA N-terminal domain dynamics to prevent DNA binding.靶向MarA N端结构域动态变化以阻止DNA结合。
Protein Sci. 2025 Jan;34(1):e5258. doi: 10.1002/pro.5258.
4
Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor.研究与工程一种 1,2-丙二醇响应型转录因子生物传感器。
ACS Synth Biol. 2024 Jul 19;13(7):2177-2187. doi: 10.1021/acssynbio.4c00237. Epub 2024 Jul 5.
5
A conserved inhibitory interdomain interaction regulates DNA-binding activities of hybrid two-component systems in .一种保守的抑制性结构域间相互作用调节了……中杂合双组分系统的DNA结合活性。
mBio. 2024 Jul 17;15(7):e0122024. doi: 10.1128/mbio.01220-24. Epub 2024 Jun 6.
6
Role of Natural Binding Proteins in Therapy and Diagnostics.天然结合蛋白在治疗与诊断中的作用。
Life (Basel). 2024 May 15;14(5):630. doi: 10.3390/life14050630.
7
AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4.阿拉伯糖胞苷转录调节因子、天冬氨酸半醛脱氢酶和酰基转移酶：不动杆菌属菌株DF4苯酚分解代谢途径中的三个假定基因。
J Genet Eng Biotechnol. 2024 Mar;22(1):100349. doi: 10.1016/j.jgeb.2023.100349. Epub 2024 Jan 22.
8
Determining the potential targets of silybin by molecular docking and its antibacterial functions on efflux pumps and porins in uropathogenic E. coli.通过分子对接确定水飞蓟宾的潜在靶点及其对尿路致病性大肠杆菌中流出泵和孔蛋白的抗菌功能。
Int Microbiol. 2025 May;28(Suppl 1):63-74. doi: 10.1007/s10123-024-00488-9. Epub 2024 Feb 16.
9
Suppressors of Mutation Identify New Regulators of LpxC, Which Mediates the First Committed Step in Lipopolysaccharide Biosynthesis.突变抑制因子鉴定出 LPS 生物合成第一步中 LpxC 的新调节因子。
Int J Mol Sci. 2023 Oct 14;24(20):15174. doi: 10.3390/ijms242015174.
10
The Mar, Sox, and Rob Systems.马尔、索克斯和罗布系统。
EcoSal Plus. 2023 Dec 12;11(1):eesp00102022. doi: 10.1128/ecosalplus.esp-0010-2022. Epub 2023 Apr 4.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Collaborative Computational Project, number 4: providing programs for protein crystallography.合作计算项目4：提供蛋白质晶体学程序。
Methods Enzymol. 1997;277:620-33. doi: 10.1016/s0076-6879(97)77034-4.
3
New parameters for the refinement of nucleic acid-containing structures.用于优化含核酸结构的新参数。
Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):57-64. doi: 10.1107/S0907444995011115.
4
Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility.作为二聚体与DNA结合的Pit-1 POU结构域的结构：意外的排列和灵活性。
Genes Dev. 1997 Jan 15;11(2):198-212. doi: 10.1101/gad.11.2.198.
5
Structural classification of HTH DNA-binding domains and protein-DNA interaction modes.螺旋-转角-螺旋DNA结合结构域的结构分类及蛋白质-DNA相互作用模式。
J Mol Biol. 1996 Sep 20;262(2):294-313. doi: 10.1006/jmbi.1996.0514.
6
Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli.大肠杆菌中MarA转录激活因子对marRAB多抗生素抗性操纵子的自激活作用。
J Bacteriol. 1996 Apr;178(8):2216-23. doi: 10.1128/jb.178.8.2216-2223.1996.
7
The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA.与端粒DNA结合的酵母RAP1的DNA结合结构域的晶体结构。
Cell. 1996 Apr 5;85(1):125-36. doi: 10.1016/s0092-8674(00)81088-0.
8
Interactions of protein antigens with antibodies.蛋白质抗原与抗体的相互作用。
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):7-12. doi: 10.1073/pnas.93.1.7.
9
Purification and regulatory properties of MarA protein, a transcriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters.大肠杆菌多重抗生素和超氧化物抗性启动子的转录激活因子MarA蛋白的纯化及调控特性
J Bacteriol. 1995 Dec;177(24):7100-4. doi: 10.1128/jb.177.24.7100-7104.1995.
10
The XylS/AraC family of regulators.XylS/AraC 调节因子家族。
Nucleic Acids Res. 1993 Feb 25;21(4):807-10. doi: 10.1093/nar/21.4.807.
Zotero 插件