Rhee S, Martin R G, Rosner J L, Davies D R
Laboratory of Molecular Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0560, USA.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10413-8. doi: 10.1073/pnas.95.18.10413.
A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.
本文描述了原核生物转录激活因子AraC家族成员MarA与其同源DNA结合位点形成的晶体结构。MarA由两个相似的亚结构域组成，每个亚结构域都包含一个螺旋-转角-螺旋DNA结合基序。基序中的两个识别螺旋插入到DNA同一面上相邻的大沟片段中，但仅相隔27埃，从而使DNA弯曲约35度。识别螺旋与DNA大沟之间广泛的相互作用提供了序列特异性。
