Berrier A, Siu G, Calame K
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
J Immunol. 1998 Sep 1;161(5):2267-75.
NF-IL6 is an important transcriptional regulator of genes induced in activated monocytes/macrophages, and NF-IL6 is the only CCAAT/enhancer-binding protein (C/EBP) family member whose steady-state mRNA levels increase upon activation of monocytes (1). We show that increased transcription of the NF-IL6 gene is responsible, at least in part, for induction of NF-IL6 mRNA following activation of U937 promonocytic cells. We have identified a 104-bp minimal promoter region of the NF-IL6 gene that is sufficient for basal and activation-dependent induction of transcription in U937 cells. This region contains binding sites for the cAMP response element-binding protein/activation transcription factor (CREB/ATF) and Sp1 families of transcription factors. Each site is functionally important and contributes independently to transcription of the NF-IL6 gene in U937 cells.
NF-IL6是活化单核细胞/巨噬细胞中诱导基因的重要转录调节因子，并且NF-IL6是唯一一种在单核细胞活化后其稳态mRNA水平会升高的CCAAT/增强子结合蛋白（C/EBP）家族成员（1）。我们发现，NF-IL6基因转录的增加至少部分地导致了U937原单核细胞活化后NF-IL6 mRNA的诱导。我们已经鉴定出NF-IL6基因的一个104bp最小启动子区域，该区域足以在U937细胞中进行基础转录和依赖于活化的转录诱导。该区域包含cAMP反应元件结合蛋白/活化转录因子（CREB/ATF）和Sp1转录因子家族的结合位点。每个位点在功能上都很重要，并独立地促进U937细胞中NF-IL6基因的转录。
