Nakase K, Sartor M
Department of Haematology, Westmead Hospital, New South Wales, Australia.
Cytometry. 1998 Aug 15;34(4):198-202. doi: 10.1002/(sici)1097-0320(19980815)34:4<198::aid-cyto4>3.0.co;2-c.
The value of flow cytometric detection of myeloperoxidase (MPO) in the differential diagnosis of acute leukemia was evaluated in 57 cases of acute leukemia and in 9 leukemia cell lines. Cells were fixed and permeabilized with Fix & Perm cell permeabilization kit at room temperature for 15 min each, and stained with anti-MPO monoclonal antibody (MPO-7) by direct immunofluorescence. One myeloid cell line, HL-60, was MPO-positive, while the other myeloid cell lines (KG-1, K-562, and MEG-01) as well as lymphoid cell lines (KM-3, NALM-6, Raji, REH, and T-ALL-1) were MPO-negative as previously described. Among acute leukemias, MPO was detected in 23 of 26 cases of acute myeloid leukemia (AML), 7 of 23 cases of B-lineage acute lymphoblastic leukemia (ALL), 1 of 6 cases of T-lineage ALL (T-ALL), and 1 of 2 cases of acute unclassified leukemia (AUL). The intensity of MPO expression in 6 of 7 B-lineage ALL cases was weak compared with AML labeling. There was no detectable cytochemical MPO in the cells of ALL, AUL, or AML that stained negative for anti-MPO. No relationship between the expression of MPO and myeloid lineage surface antigens was observed in ALL. Three cases of MPO-positive ALL and AUL could be reclassified as biphenotypic leukemia according to the revised Catovsky scoring system. These results indicate that anti-MPO is an excellent marker for the diagnosis and classification of acute leukemia and can be reliably detected by flow cytometry. This rapid technique should be a valuable addition to routine immunophenotyping of acute leukemia.
在57例急性白血病患者和9种白血病细胞系中评估了流式细胞术检测髓过氧化物酶（MPO）在急性白血病鉴别诊断中的价值。细胞在室温下用Fix & Perm细胞通透化试剂盒固定并通透化，每次15分钟，然后通过直接免疫荧光用抗MPO单克隆抗体（MPO-7）染色。一种髓系细胞系HL-60为MPO阳性，而其他髓系细胞系（KG-1、K-562和MEG-01）以及淋巴系细胞系（KM-3、NALM-6、Raji、REH和T-ALL-1）如前所述为MPO阴性。在急性白血病中，26例急性髓系白血病（AML）中有23例检测到MPO，23例B系急性淋巴细胞白血病（ALL）中有7例，6例T系ALL（T-ALL）中有1例，2例急性未分类白血病（AUL）中有1例。与AML标记相比，7例B系ALL病例中有6例MPO表达强度较弱。在抗MPO染色阴性的ALL、AUL或AML细胞中未检测到细胞化学MPO。在ALL中未观察到MPO表达与髓系谱系表面抗原之间的关系。根据修订的卡托夫斯基评分系统，3例MPO阳性的ALL和AUL可重新分类为双表型白血病。这些结果表明，抗MPO是急性白血病诊断和分类的优秀标志物，可通过流式细胞术可靠检测。这种快速技术应成为急性白血病常规免疫表型分析的有价值补充。
