Mandal A K, Walia R
Department of Pathology, Maulana Azad Medical College, New Delhi.
Indian J Pathol Microbiol. 1995 Oct;38(4):403-6.
This experimental work was undertaken to study the difference in cell growth due to different attachment surface. Three types of attachment surface were studied (i) collagenized surface (ii) glass surface and (iii) plastic surface. Rat tail collagen suspension was prepared and coated on culture flasks. Human breast epithelial cells and breast carcinoma cells were cultured for three weeks. Cell counts were made before and after one, two and three weeks of culture. Out of five cases only two survived for more than a week. The best survival was observed on plastic surface. Collagenized and glass surfaces had similar results. Thus plastic surface is probably the best compared to glass and collagenized surface, possibly because the plastic surface provides better adhesion to malignant cells. It was also seen that in plastic surface the cells get lined up around a basement membrane like structure growing a resemblance to ductal structure. This is contradictory to the prevalent view that collagenized surface is best for in vitro growth and differentiation.
进行这项实验工作是为了研究不同附着表面对细胞生长的影响。研究了三种附着表面：（i）胶原化表面；（ii）玻璃表面；（iii）塑料表面。制备大鼠尾胶原悬液并包被于培养瓶。人乳腺上皮细胞和乳腺癌细胞培养三周。在培养1周、2周和3周前后进行细胞计数。5例中只有2例存活超过1周。在塑料表面观察到最佳的细胞存活情况。胶原化表面和玻璃表面的结果相似。因此，与玻璃表面和胶原化表面相比，塑料表面可能是最佳选择，这可能是因为塑料表面能为恶性细胞提供更好的黏附性。还观察到在塑料表面，细胞围绕类似基底膜的结构排列成行，逐渐形成类似于导管的结构。这与普遍认为胶原化表面最有利于体外生长和分化的观点相矛盾。
