一氧化氮（NO）/环磷酸鸟苷（cGMP）信号通路在豚鼠前庭系统中的定位。

Localisation of the nitric oxide (NO)/cGMP-pathway in the vestibular system of guinea pigs.

作者信息

Hess A, Bloch W, Su J, Stennert E, Addicks K, Michel O

机构信息

Department of Oto-Rhino-Laryngology, University of Cologne, Germany.

出版信息

Neurosci Lett. 1998 Jul 31;251(3):185-8. doi: 10.1016/s0304-3940(98)00532-1.

DOI:10.1016/s0304-3940(98)00532-1

PMID:9726374

Abstract

The exact distribution of nitric oxide-synthases (NOS) in the vestibular system has not been described satisfying yet. Immunostaining, using specific antibodies to the three known NOS-isoforms, to cyclic guanosine monophosphate (cGMP) and soluble guanylyl-cyclase (sGC), the second messenger system of nitric oxide (NO), was performed on paraffin sections of temporal bone from guinea pigs. eNOS could be detected in vestibular ganglion cells and in nerve fibres, including the calyces, surrounding the type 1 hair cells (HC). bNOS was found in the sensory epithelium, ganglion cells and in bone, while iNOS could not be found. NOS-detection was accompanied by reactivity to sGC and to cGMP. This finding implies that b- and eNOS-generated NO is involved in regulative processes in neurotransmission and regulation of blood flow.

摘要

一氧化氮合酶（NOS）在前庭系统中的精确分布尚未得到令人满意的描述。使用针对三种已知NOS同工型、环磷酸鸟苷（cGMP）和可溶性鸟苷酸环化酶（sGC）（一氧化氮（NO）的第二信使系统）的特异性抗体，对豚鼠颞骨石蜡切片进行免疫染色。在内耳前庭神经节细胞和神经纤维中，包括围绕I型毛细胞（HC）的壶腹，可以检测到内皮型一氧化氮合酶（eNOS）。在感觉上皮、神经节细胞和骨中发现了脑型一氧化氮合酶（bNOS），而未发现诱导型一氧化氮合酶（iNOS）。NOS检测伴随着对sGC和cGMP的反应性。这一发现表明，bNOS和eNOS产生的NO参与神经传递和血流调节过程。