紫杉醇（泰素）与放疗对喉鳞状细胞癌细胞系的细胞毒性和遗传毒性作用

Cytotoxic and genotoxic effects of paclitaxel (Taxol) and radiation in a squamous cell carcinoma cell line of the larynx.

作者信息

Preisler V K, Stopper H, Schindler D, Friedl R, Pfreundner L, Hoppe F, Hagen R

机构信息

Department of Otorhinolaryngology, University of Würzburg, Germany.

出版信息

Acta Otolaryngol. 1998 Jul;118(4):600-5. doi: 10.1080/00016489850154793.

DOI:10.1080/00016489850154793

PMID:9726690

Abstract

Paclitaxel (Taxol) is an antimicrotubular agent which blocks the cells in the G2/M phase of the cell cycle. Because of this mechanism it is presumed that this drug could function as a radiation sensitizer. The cytotoxic and genotoxic effects of paclitaxel and a combination of paclitaxel and radiation were studied in the human laryngeal carcinoma cell line HLac 79. The growth of the cells was significantly reduced at concentrations of paclitaxel as low as 10 nM. Flow cytometry data showed a G2/M block after exposure to paclitaxel. Radiation at 12 and 24 h after drug treatment exerted an additive but no radiation sensitizing effect. As genotoxic effect paclitaxel induced multinucleated cells, possibly in a synergistic manner, at low concentrations (10 nM) and radiation doses up to 3 Gy.

摘要

紫杉醇（泰素）是一种抗微管药物，可使细胞周期阻滞于G2/M期。基于这一机制，推测该药物可作为放射增敏剂。研究了紫杉醇以及紫杉醇与放疗联合应用对人喉癌细胞系HLac 79的细胞毒性和遗传毒性作用。当紫杉醇浓度低至10 nM时，细胞生长即显著受到抑制。流式细胞术数据显示，紫杉醇处理后出现G2/M期阻滞。药物处理后12小时和24小时进行放疗，显示出相加作用，但无放射增敏效应。作为遗传毒性效应，紫杉醇在低浓度（10 nM）和高达3 Gy的辐射剂量下，可能以协同方式诱导多核细胞形成。