De Wever H, Vereecken K, Stolz A, Verachtert H
Laboratory for Industrial Microbiology and Biochemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium.
Appl Environ Microbiol. 1998 Sep;64(9):3270-4. doi: 10.1128/AEM.64.9.3270-3274.1998.
Benzothiazole-2-sulfonate (BTSO3) is one of the side products occurring in 2-mercaptobenzothiazole (MBT) production wastewater. We are the first to isolate an axenic culture capable of BTSO3 degradation. The isolate was identified as a Rhodococcus erythropolis strain and also degraded 2-hydroxybenzothiazole (OBT) and benzothiazole (BT), but not MBT, which was found to inhibit the biodegradation of OBT, BT, and BTSO3. In anaerobic resting cell assays, BTSO3 was transformed into OBT in stoichiometric amounts. Under aerobic conditions, OBT was observed as an intermediate in BT breakdown and an unknown compound transiently accumulated in several assays. This product was identified as a dihydroxybenzothiazole. Benzothiazole degradation pathways seem to converge into OBT, which is then transformed further into the dihydroxy derivative.
苯并噻唑 - 2 - 磺酸盐（BTSO3）是2 - 巯基苯并噻唑（MBT）生产废水中产生的副产物之一。我们首次分离出一种能够降解BTSO3的纯培养物。该分离物被鉴定为红平红球菌菌株，它还能降解2 - 羟基苯并噻唑（OBT）和苯并噻唑（BT），但不能降解MBT，发现MBT会抑制OBT、BT和BTSO3的生物降解。在厌氧静息细胞试验中，BTSO3以化学计量的量转化为OBT。在有氧条件下，OBT是BT分解过程中的中间产物，并且在几次试验中一种未知化合物短暂积累。该产物被鉴定为二羟基苯并噻唑。苯并噻唑的降解途径似乎汇聚到OBT，然后OBT进一步转化为二羟基衍生物。
