De Wever H, De Moor K, Verachtert H
Catholic University of Leuven, Laboratory for Industrial Microbiology and Biochemistry, Heverlee, Belgium.
Appl Microbiol Biotechnol. 1994 Dec;42(4):631-5. doi: 10.1007/BF00173931.
Active sludge systems containing benzothiazoles may be intoxicated by 2-mercaptobenzothiazole (MBT). This toxicity towards several bacteria is now confirmed and is situated at around 100 mg MBT l-1. Octanol-water partition coefficients indicated that MBT might interact with membrane-bound systems. This was confirmed through experiments showing that bacterial cell respiration was inhibited using lactate or succinate as substrates. Using these substrates and also NADH, it was found that their oxidation was also inhibited using isolated membrane fragments of Escherichia coli and Paracoccus denitrificans. Methylene blue reduction was also found to be inhibited. The oxidation of ascorbate was not inhibited in P. denitrificans. From these results it is suggested that MBT might interact with the respiratory chain at the level of flavoproteins or quinones and Fe-S clusters.
含有苯并噻唑的活性污泥系统可能会被2-巯基苯并噻唑（MBT）毒害。现在已证实MBT对几种细菌具有这种毒性，其毒性阈值约为100 mg MBT l-1。正辛醇-水分配系数表明MBT可能与膜结合系统相互作用。通过实验证实了这一点，实验表明以乳酸盐或琥珀酸盐为底物时细菌细胞呼吸受到抑制。使用这些底物以及NADH时，发现用大肠杆菌和反硝化副球菌的分离膜片段时它们的氧化也受到抑制。还发现亚甲基蓝还原受到抑制。在反硝化副球菌中抗坏血酸盐的氧化未受到抑制。从这些结果表明，MBT可能在黄素蛋白、醌或铁硫簇水平与呼吸链相互作用。
