Cercignani G, Lucia S, Petracchi D
Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
Biophys J. 1998 Sep;75(3):1466-72. doi: 10.1016/S0006-3495(98)74065-2.
Halobacterium salinarum cells from 3-day-old cultures have been stimulated with different patterns of repetitive pulse stimuli. A short train of 0.6-s orange light pulses with a 4-s period resulted in reversal peaks of increasing intensity. The reverse occurred when blue light pulses were delivered as a finite train: with a 3-s period, the response declined in sequence from the first to the last pulse. To evaluate the response of the system under steady-state conditions of stimulation, continuous trains of pulses were also applied; whereas blue light always produced a sharply peaked response immediately after each pulse, orange pulses resulted in a declining peak of reversals that lasted until the subsequent pulse. An attempt to account for these results in terms of current excitation/adaptation models shows that additional mechanisms appear to be at work in this transduction chain.
来自3日龄培养物的盐生盐杆菌细胞已受到不同模式的重复脉冲刺激。一系列持续0.6秒、周期为4秒的橙色光脉冲导致强度不断增加的反转峰。当蓝光脉冲以有限序列施加时情况则相反：周期为3秒时，响应从第一个脉冲到最后一个脉冲依次下降。为了评估该系统在稳态刺激条件下的响应，也施加了连续的脉冲序列；蓝光总是在每个脉冲后立即产生一个尖锐的峰值响应，而橙色脉冲则导致反转峰下降，这种下降一直持续到后续脉冲。试图根据当前的兴奋/适应模型来解释这些结果表明，在这个转导链中似乎还有其他机制在起作用。
