流式细胞术原位聚合酶链反应作为一种检测HIV-1前病毒DNA的创新方法。
Flow cytometry in situ polymerase chain reaction as an innovative approach to HIV-1 proviral DNA detection.
作者信息
Gibellini D, Zauli G, Re M C, Furlini G, La Placa M
机构信息
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
出版信息
Eur J Histochem. 1998;42(2):137-41.
In situ polymerase chain reaction represents an intriguing method to couple the sensitivity of PCR technology with the preservation of cell morphology. The target of this technique is the detection of specific DNA or retrotranscribed RNA inside the cell. In this brief report, we describe an in situ polymerase chain reaction technique revealed by flow cytometry in order to reveal the human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells. We also discuss the key steps and parameters of our assay in comparison with current opinion on in situ polymerase chain reaction.
原位聚合酶链反应是一种将聚合酶链反应（PCR）技术的敏感性与细胞形态的保存相结合的有趣方法。该技术的目标是检测细胞内的特定DNA或逆转录RNA。在本简短报告中，我们描述了一种通过流式细胞术揭示的原位聚合酶链反应技术，以揭示外周血单核细胞中的1型人类免疫缺陷病毒（HIV-1）前病毒DNA。我们还将我们检测方法的关键步骤和参数与当前关于原位聚合酶链反应的观点进行了比较讨论。
Zotero 插件