Abadi J, Friedman J, Mageed R A, Jefferis R, Rodriguez-Barradas M C, Pirofski L
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
J Infect Dis. 1998 Sep;178(3):707-16. doi: 10.1086/515369.
Human immunodeficiency virus (HIV)-infected persons manifest decreased antibody responses to pneumococcal polysaccharide vaccines. Since human antibody responses to polysaccharides are often restricted, the molecular structure of antibodies elicited by a 23-valent pneumococcal vaccine was analyzed. Anti-idiotypic reagents were used to detect V(H)1, V(H)3, and V(H)4 gene usage by antibodies to pneumococcal capsular polysaccharides in HIV-uninfected and HIV-infected subjects by ELISA. HIV-uninfected persons generated beta-mercaptoethanol-sensitive and -resistant antibodies to pneumococcal capsular polysaccharides expressing V(H)3 determinants recognized by the D12, 16.84, and B6 monoclonal antibodies; antibodies expressing V(H)1 determinants were not detected, and V(H)4 determinants were expressed by beta-mercaptoethanol-sensitive antibodies only; and HIV-infected subjects had significantly lower capsular polysaccharide-specific and V(H)3-positive antibody responses. These findings confirm decreased antibody responses to pneumococcal vaccination in HIV-infected persons and suggest that their poor responses may result from HIV-associated depletion of restricted B cell subsets.
感染人类免疫缺陷病毒（HIV）的个体对肺炎球菌多糖疫苗的抗体反应降低。由于人类对多糖的抗体反应通常受到限制，因此对23价肺炎球菌疫苗引发的抗体的分子结构进行了分析。通过酶联免疫吸附测定（ELISA），使用抗独特型试剂检测未感染HIV和感染HIV的受试者中针对肺炎球菌荚膜多糖的抗体对V(H)1、V(H)3和V(H)4基因的使用情况。未感染HIV的个体产生了对表达由D12、16.84和B6单克隆抗体识别的V(H)3决定簇的肺炎球菌荚膜多糖的β-巯基乙醇敏感和抗性抗体；未检测到表达V(H)1决定簇的抗体，且V(H)4决定簇仅由β-巯基乙醇敏感抗体表达；而感染HIV的受试者的荚膜多糖特异性和V(H)3阳性抗体反应明显较低。这些发现证实了感染HIV的个体对肺炎球菌疫苗的抗体反应降低，并表明其反应不佳可能是由于HIV相关的受限B细胞亚群耗竭所致。
