从感染和未感染人类免疫缺陷病毒的儿童中分离出的鸟分枝杆菌复合群菌株的遗传多样性。

Genetic diversity among Mycobacterium avium complex strains recovered from children with and without human immunodeficiency virus infection.

作者信息

Swanson D S, Pan X, Kline M W, McKinney R E, Yogev R, Lewis L L, Brady M T, McSherry G D, Dankner W M, Musser J M

机构信息

Department of Pediatrics, and Institute for the Study of Human Bacterial Pathogenesis, Baylor College of Medicine, Houston, Texas 77030, USA.

出版信息

J Infect Dis. 1998 Sep;178(3):776-82. doi: 10.1086/515364.

DOI:10.1086/515364

PMID:9728547

Abstract

The genetic diversity and molecular epidemiology of Mycobacterium avium complex (MAC) infections in children with and without human immunodeficiency virus (HIV) infection were evaluated. Isolates recovered from 136 children were subtyped by sequence analysis of a 360-bp region of the gene (hsp65) encoding a 65-kDa heat-shock protein. Twenty-one distinct hsp65 alleles were identified. On the basis of hsp65 genotype, 6 isolates were not MAC organisms. Of the remaining 130 samples, 61% were M. avium, 37% were Mycobacterium intracellulare, and 2% were species nonspecific MAC. Eighty-eight percent of the isolates obtained from HIV-infected children were M. avium. In contrast, only 38% of the isolates obtained from children without HIV infection were M. avium (chi2 test, P < .001). M. avium isolates were further subtyped by Southern blot analysis with insertion element IS1245. Taken together, no evidence for a single clonal M. avium strain causing infection was detected.

摘要

对感染和未感染人类免疫缺陷病毒（HIV）的儿童鸟分枝杆菌复合群（MAC）感染的遗传多样性和分子流行病学进行了评估。从136名儿童中分离出的菌株，通过对编码65 kDa热休克蛋白的基因（hsp65）的360 bp区域进行序列分析来进行亚型分型。鉴定出21种不同的hsp65等位基因。根据hsp65基因型，6株菌株不是MAC微生物。在其余130个样本中，61%为鸟分枝杆菌，37%为胞内分枝杆菌，2%为非特异性MAC菌种。从感染HIV的儿童中分离出的菌株88%为鸟分枝杆菌。相比之下，从未感染HIV的儿童中分离出的菌株只有38%是鸟分枝杆菌（卡方检验，P <.001）。通过插入元件IS1245的Southern印迹分析对鸟分枝杆菌菌株进一步进行亚型分型。综合来看，未检测到单一克隆性鸟分枝杆菌菌株引起感染的证据。