Jouault T, Fradin C, Trinel P A, Bernigaud A, Poulain D
Unité INSERM 42, Domaine du CERTIA, Villeneuve d'Ascq, France.
J Infect Dis. 1998 Sep;178(3):792-802. doi: 10.1086/515361.
Cell wall beta-1,2-oligomannosides are involved in Candida albicans binding to macrophages and in their stimulation to produce cytokines. The nature of signaling events occurring during initial interaction of macrophage J774 cell line and C. albicans, together with the nature of molecules containing beta-1,2-oligomannosides released by the yeasts, was examined. Cocultivation led to a herbimycin A-sensitive production of tumor necrosis factor-alpha. Immunofluorescence and Western blotting confirmed tyrosine phosphorylation and revealed an accumulation of 90- to 120-kDa phosphoproteins. Antibodies specific for beta-1,2-oligomannosides showed that these epitopes were shed at an early stage from the yeasts to the macrophage membrane, in association with a glycolipid previously described as C. albicans phospholipomannan. Incubation of macrophages with purified phospholipomannan alone led to a signal transduction pathway identical to that observed with living yeasts. All of these results demonstrate that C. albicans phospholipomannan shedding is involved in C. albicans-macrophage interaction through beta-1,2-oligomannosides.
细胞壁β-1,2-寡聚甘露糖参与白色念珠菌与巨噬细胞的结合以及刺激巨噬细胞产生细胞因子。研究了巨噬细胞J774细胞系与白色念珠菌初始相互作用期间发生的信号转导事件的性质，以及酵母释放的含β-1,2-寡聚甘露糖分子的性质。共培养导致对赫曲霉素A敏感的肿瘤坏死因子-α的产生。免疫荧光和蛋白质印迹证实了酪氨酸磷酸化，并揭示了90至120 kDa磷蛋白的积累。针对β-1,2-寡聚甘露糖的特异性抗体表明，这些表位在早期从酵母脱落到巨噬细胞膜上，与先前描述为白色念珠菌磷脂甘露聚糖的糖脂相关。仅用纯化的磷脂甘露聚糖孵育巨噬细胞会导致与活酵母观察到的相同的信号转导途径。所有这些结果表明，白色念珠菌磷脂甘露聚糖的脱落通过β-1,2-寡聚甘露糖参与白色念珠菌与巨噬细胞的相互作用。
