经派伊尔结对兔进行DNA疫苗接种后，针对C4/V3构建体的黏膜和全身抗体反应。

Mucosal and systemic antibody responses to a C4/V3 construct following DNA vaccination of rabbits via the Peyer's patch.

作者信息

Winchell J M, Routray S, Betts P W, Van Kruiningen H J, Silbart L K

机构信息

University of Connecticut, Department of Molecular and Cell Biology, Storrs 06269-4039, USA.

出版信息

J Infect Dis. 1998 Sep;178(3):850-3. doi: 10.1086/515341.

DOI:10.1086/515341

PMID:9728557

Abstract

A plasmid encoding T1-SP10MN(A), a peptide derived from immunodominant regions of human immunodeficiency virus type 1 gp120, was delivered to rabbit Peyer's patches using a helium-driven gene gun. Six weeks thereafter, 2 of 5 animals were given an intradermal booster immunization. Blood, feces, and vaginal washes were collected weekly and assayed by ELISA. High titer T1-SP10MN(A)-specific fecal and vaginal secretory IgA responses were observed, and the response appeared to be augmented following dermal booster immunizations. Specific serum IgG was also detected within 1 week of immunization and remained elevated through week 20 in the 2 animals receiving dermal boosts (titers > or = 6400). This study establishes the Peyer's patch as a promising target tissue for DNA vaccination and demonstrates the efficacy of gene gun-mediated delivery of foreign DNA to a mucosal tissue for the induction of an immune response.

摘要

一种编码T1-SP10MN(A)（一种源自人类免疫缺陷病毒1型gp120免疫显性区域的肽）的质粒，通过氦驱动基因枪递送至兔派伊尔结。六周后，对5只动物中的2只进行皮内加强免疫。每周收集血液、粪便和阴道冲洗液，并通过酶联免疫吸附测定法进行检测。观察到高滴度的T1-SP10MN(A)特异性粪便和阴道分泌型IgA反应，并且在皮内加强免疫后反应似乎增强。在免疫后1周内也检测到特异性血清IgG，并且在接受皮内加强免疫的2只动物中，直到第20周IgG水平一直升高（滴度≥6400）。本研究确立了派伊尔结作为DNA疫苗接种的一个有前景的靶组织，并证明了基因枪介导的外源DNA递送至黏膜组织以诱导免疫反应的有效性。