Benestad H B, Sørensen T, Iranpour K M, Liestøl K, Yogesan K, Strøm-Gundersen I, Wang X, Løvhaug D
Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Norway.
Exp Hematol. 1998 Sep;26(10):936-41.
A system for automatic analysis of in vitro hematopoietic colonies is described and evaluated. With the standard resolution provided by video cameras, the improvement in visualization obtained using features other than size and darkness when classifying potential colonies appears to be limited. We confirmed this by comparing results obtained with the test system with those obtained with a commercial one. However, for some applications it may be useful to supplement the system with specific methods, e.g., to separate merged colonies. Digital image analyses provide new possibilities, for instance of measuring the total cellularity of the dish or analyzing colonies according to the size and cell density of each colony. Examples provided are time course studies of colony development, cellularity feedback effects on colony sizes, and bell-shaped dose-response curves for the growth stimulation obtained by certain conditioned media on a subpopulation of progenitor cells that gives rise to large colonies.
本文描述并评估了一种用于体外造血集落自动分析的系统。在摄像机提供的标准分辨率下，在对潜在集落进行分类时，使用除大小和明暗度之外的特征所获得的可视化效果改善似乎有限。我们通过将测试系统获得的结果与商业系统获得的结果进行比较来证实了这一点。然而，对于某些应用，用特定方法补充该系统可能会很有用，例如分离融合的集落。数字图像分析提供了新的可能性，例如测量培养皿中的总细胞数或根据每个集落的大小和细胞密度分析集落。所提供的例子包括集落发育的时间进程研究、细胞数对集落大小的反馈效应，以及某些条件培养基对产生大型集落的祖细胞亚群的生长刺激所获得的钟形剂量反应曲线。
